{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Sun T"],"pubmed_abstract":["Salt stress caused by soil salinization, is one of the main factors that reduce soybean yield and quality. A large number of genes have been found to be involved in the regulation of salt tolerance. In this study, we characterized a soybean sodium/hydrogen exchanger gene <i>GmNHX5</i> and revealed its functional mechanism involved in the salt tolerance process in soybean. <i>GmNHX5</i> responded to salt stress at the transcription level in the salt stress-tolerant soybean plants, but not significantly changed in the salt-sensitive ones. GmNHX5 was located in the Golgi apparatus, and distributed in new leaves and vascular, and was induced by salt treatment. Overexpression of <i>GmNHX5</i> improved the salt tolerance of hairy roots induced by soybean cotyledons, while the opposite was observed when <i>GmNHX5</i> was knockout by CRISPR/Cas9. Soybean seedlings overexpressing <i>GmNHX5</i> also showed an increased expression of <i>GmSOS1</i>, <i>GmSKOR</i>, and <i>GmHKT1</i>, higher K<sup>+</sup>/Na<sup>+</sup> ratio, and higher viability when exposed to salt stress. Our findings provide an effective candidate gene for the cultivation of salt-tolerant germplasm resources and new clues for further understanding of the salt-tolerance mechanism in plants."],"journal":["Frontiers in plant science"],"pagination":["638340"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7985447"],"repository":["biostudies-literature"],"pubmed_title":["A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K<sup>+</sup>/Na<sup>+</sup> Ratio in Soybean."],"pmcid":["PMC7985447"],"pubmed_authors":["Wang M","Fan H","Zhang J","Ma N","Wang C","Cao J","Wang D","Sun T"],"additional_accession":[]},"is_claimable":false,"name":"A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K<sup>+</sup>/Na<sup>+</sup> Ratio in Soybean.","description":"Salt stress caused by soil salinization, is one of the main factors that reduce soybean yield and quality. A large number of genes have been found to be involved in the regulation of salt tolerance. In this study, we characterized a soybean sodium/hydrogen exchanger gene <i>GmNHX5</i> and revealed its functional mechanism involved in the salt tolerance process in soybean. <i>GmNHX5</i> responded to salt stress at the transcription level in the salt stress-tolerant soybean plants, but not significantly changed in the salt-sensitive ones. GmNHX5 was located in the Golgi apparatus, and distributed in new leaves and vascular, and was induced by salt treatment. Overexpression of <i>GmNHX5</i> improved the salt tolerance of hairy roots induced by soybean cotyledons, while the opposite was observed when <i>GmNHX5</i> was knockout by CRISPR/Cas9. Soybean seedlings overexpressing <i>GmNHX5</i> also showed an increased expression of <i>GmSOS1</i>, <i>GmSKOR</i>, and <i>GmHKT1</i>, higher K<sup>+</sup>/Na<sup>+</sup> ratio, and higher viability when exposed to salt stress. Our findings provide an effective candidate gene for the cultivation of salt-tolerant germplasm resources and new clues for further understanding of the salt-tolerance mechanism in plants.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-04-30T03:11:35.564Z","creation":"2025-04-05T19:12:20.308Z"},"accession":"S-EPMC7985447","cross_references":{"pubmed":["33767722"],"doi":["10.3389/fpls.2021.638340"]}}