{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Meng S"],"funding":["NEI NIH HHS","NHLBI NIH HHS","NIGMS NIH HHS","NIH HHS"],"pagination":["1647-1662"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7987209"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["142(17)"],"pubmed_abstract":["<h4>Background</h4>The angiogenic response to ischemia restores perfusion so as to preserve tissue. A role for mesenchymal-to-endothelial transition in the angiogenic response is controversial. This study is to determine if resident fibroblasts contribute to angiogenesis.<h4>Methods</h4>We utilized the murine model of hindlimb ischemia, and in vivo Matrigel plug assay together with lineage tracing studies and single cell RNA-sequencing to examine the transcriptional and functional changes in fibroblasts in response to ischemia.<h4>Results</h4>Lineage tracing using Fsp1-Cre: R26R-EYFP mice revealed the emergence within the ischemic hindlimb of a small subset of YFP<sup>+</sup> CD144<sup>+</sup> CD11b<sup>-</sup> fibroblasts (E* cells) that expressed endothelial cell (EC) genes. Subcutaneous"],"journal":["Circulation"],"pubmed_title":["Reservoir of Fibroblasts Promotes Recovery From Limb Ischemia."],"pmcid":["PMC7987209"],"funding_grant_id":["R01 HL148338","R01 HL133254","S10 OD018033","R01 GM125632","P30 EY002520","S10 OD023469"],"pubmed_authors":["Lv J","Meng S","Chen K","Cooke JP","Owusu I","Chanda PK"],"additional_accession":[]},"is_claimable":false,"name":"Reservoir of Fibroblasts Promotes Recovery From Limb Ischemia.","description":"<h4>Background</h4>The angiogenic response to ischemia restores perfusion so as to preserve tissue. A role for mesenchymal-to-endothelial transition in the angiogenic response is controversial. This study is to determine if resident fibroblasts contribute to angiogenesis.<h4>Methods</h4>We utilized the murine model of hindlimb ischemia, and in vivo Matrigel plug assay together with lineage tracing studies and single cell RNA-sequencing to examine the transcriptional and functional changes in fibroblasts in response to ischemia.<h4>Results</h4>Lineage tracing using Fsp1-Cre: R26R-EYFP mice revealed the emergence within the ischemic hindlimb of a small subset of YFP<sup>+</sup> CD144<sup>+</sup> CD11b<sup>-</sup> fibroblasts (E* cells) that expressed endothelial cell (EC) genes. Subcutaneous","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-04T18:41:58.864Z","creation":"2025-04-04T18:41:58.864Z"},"accession":"S-EPMC7987209","cross_references":{"pubmed":["32820662"],"doi":["10.1161/CIRCULATIONAHA.120.046872","10.1161/circulationaha.120.046872"]}}