biostudies-literatureMeng SNEI NIH HHSNHLBI NIH HHSNIGMS NIH HHSNIH HHS1647-1662https://www.ebi.ac.uk/biostudies/studies/S-EPMC7987209biostudies-literatureUnknown142(17)<h4>Background</h4>The angiogenic response to ischemia restores perfusion so as to preserve tissue. A role for mesenchymal-to-endothelial transition in the angiogenic response is controversial. This study is to determine if resident fibroblasts contribute to angiogenesis.<h4>Methods</h4>We utilized the murine model of hindlimb ischemia, and in vivo Matrigel plug assay together with lineage tracing studies and single cell RNA-sequencing to examine the transcriptional and functional changes in fibroblasts in response to ischemia.<h4>Results</h4>Lineage tracing using Fsp1-Cre: R26R-EYFP mice revealed the emergence within the ischemic hindlimb of a small subset of YFP⁺ CD144⁺ CD11b^- fibroblasts (E* cells) that expressed endothelial cell (EC) genes. SubcutaneousCirculationReservoir of Fibroblasts Promotes Recovery From Limb Ischemia.PMC7987209R01 HL148338R01 HL133254S10 OD018033R01 GM125632P30 EY002520S10 OD023469Lv JMeng SChen KCooke JPOwusu IChanda PKfalseReservoir of Fibroblasts Promotes Recovery From Limb Ischemia.<h4>Background</h4>The angiogenic response to ischemia restores perfusion so as to preserve tissue. A role for mesenchymal-to-endothelial transition in the angiogenic response is controversial. This study is to determine if resident fibroblasts contribute to angiogenesis.<h4>Methods</h4>We utilized the murine model of hindlimb ischemia, and in vivo Matrigel plug assay together with lineage tracing studies and single cell RNA-sequencing to examine the transcriptional and functional changes in fibroblasts in response to ischemia.<h4>Results</h4>Lineage tracing using Fsp1-Cre: R26R-EYFP mice revealed the emergence within the ischemic hindlimb of a small subset of YFP⁺ CD144⁺ CD11b^- fibroblasts (E* cells) that expressed endothelial cell (EC) genes. Subcutaneous2020-01-01T00:00:00Z2020 Oct2025-04-04T18:41:58.864Z2025-04-04T18:41:58.864ZS-EPMC79872093282066210.1161/CIRCULATIONAHA.120.04687210.1161/circulationaha.120.046872