{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang Q"],"funding":["the National Natural Science Foundation of China"],"pagination":["189"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7988030"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(4)"],"pubmed_abstract":["<i>Leuconostoc citreum</i>, a type of food-grade probiotic bacteria, plays an important role in food fermentation and intestinal probiotics. Biofilms help bacteria survive under adverse conditions, and LuxS/AI-2-dependent quorum sensing (QS) plays an important role in the regulation of their biofilm-forming activities. <i>L. citreum</i> 37 was a biofilm-forming strain isolated from dairy products. The aim of this study was to analyze genes involved in the LuxS/AI-2 system based on genome sequencing and biofilm formation of <i>L. citreum</i> 37. Genome assembly yielded two contigs (one chromosome and one plasmid), and the complete genome contained 1,946,279 base pairs (bps) with a G + C content of 38.91%. The genome sequence analysis showed that there were several pathways such as the two-component system, QS, and seven other signal pathways, and 26 genes (including <i>luxS</i>, <i>pfs</i>, and 24 other genes) may participate in QS related to biofilm formation. All these results showed that the LuxS/AI-2 system is complete in the genome of <i>L. citreum</i> 37. The quantitative polymerase chain reaction (qPCR) of <i>pfs</i>, <i>luxS</i> genes, and AI-2 production of <i>L. citreum</i> 37 in planktonic state and biofilm state showed that the expression of <i>pfs</i> and <i>luxS</i> genes was consistent with the production of AI-2 and was positively correlated with biofilm formation. After <i>lux</i>S of <i>L. citreum</i> 37 expressed in <i>Escherichia coli</i> BL21, AI-2 production was detected, suggesting that the <i>luxS</i> gene played an important role in AI-2 synthesis, Therefore, <i>luxS</i> may regulate the biofilm formation of <i>L. citreum</i> 37 by participating in AI-2 synthesis. It is projected that results of this study could help facilitate further understanding and application of <i>L. citreum</i> 37.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13205-021-02747-2."],"journal":["3 Biotech"],"pubmed_title":["Research on the role of LuxS/AI-2 quorum sensing in biofilm of <i>Leuconostoc citreum</i> 37 based on complete genome sequencing."],"pmcid":["PMC7988030"],"funding_grant_id":["31860433"],"pubmed_authors":["An Q","Zhang D","Xu R","Sa R","Yang Q","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"Research on the role of LuxS/AI-2 quorum sensing in biofilm of <i>Leuconostoc citreum</i> 37 based on complete genome sequencing.","description":"<i>Leuconostoc citreum</i>, a type of food-grade probiotic bacteria, plays an important role in food fermentation and intestinal probiotics. Biofilms help bacteria survive under adverse conditions, and LuxS/AI-2-dependent quorum sensing (QS) plays an important role in the regulation of their biofilm-forming activities. <i>L. citreum</i> 37 was a biofilm-forming strain isolated from dairy products. The aim of this study was to analyze genes involved in the LuxS/AI-2 system based on genome sequencing and biofilm formation of <i>L. citreum</i> 37. Genome assembly yielded two contigs (one chromosome and one plasmid), and the complete genome contained 1,946,279 base pairs (bps) with a G + C content of 38.91%. The genome sequence analysis showed that there were several pathways such as the two-component system, QS, and seven other signal pathways, and 26 genes (including <i>luxS</i>, <i>pfs</i>, and 24 other genes) may participate in QS related to biofilm formation. All these results showed that the LuxS/AI-2 system is complete in the genome of <i>L. citreum</i> 37. The quantitative polymerase chain reaction (qPCR) of <i>pfs</i>, <i>luxS</i> genes, and AI-2 production of <i>L. citreum</i> 37 in planktonic state and biofilm state showed that the expression of <i>pfs</i> and <i>luxS</i> genes was consistent with the production of AI-2 and was positively correlated with biofilm formation. After <i>lux</i>S of <i>L. citreum</i> 37 expressed in <i>Escherichia coli</i> BL21, AI-2 production was detected, suggesting that the <i>luxS</i> gene played an important role in AI-2 synthesis, Therefore, <i>luxS</i> may regulate the biofilm formation of <i>L. citreum</i> 37 by participating in AI-2 synthesis. It is projected that results of this study could help facilitate further understanding and application of <i>L. citreum</i> 37.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13205-021-02747-2.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-05-02T22:39:45.847Z","creation":"2025-04-07T07:26:03.948Z"},"accession":"S-EPMC7988030","cross_references":{"pubmed":["33927980"],"doi":["10.1007/s13205-021-02747-2"]}}