biostudies-literatureSkocek ONICHD NIH HHS429-432https://www.ebi.ac.uk/biostudies/studies/S-EPMC7990085biostudies-literatureUnknown15(6)Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm³ at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ~15 µm and at depths up to 360 µm can be discriminated.Nature methodsHigh-speed volumetric imaging of neuronal activity in freely moving rodents.PMC7990085U54 HD087101Martinez Traub FNobauer TYamagata MCox DDXia CNGrama ASkocek OAharoni DGolshani PWeilguny LMolodtsov MIVaziri AfalseHigh-speed volumetric imaging of neuronal activity in freely moving rodents.Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm³ at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ~15 µm and at depths up to 360 µm can be discriminated.2018-01-01T00:00:00Z2018 Jun2025-04-04T12:32:36.097Z2025-02-18T23:35:57.842ZS-EPMC79900852973600010.1038/s41592-018-0008-0