{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ayuso JM"],"funding":["U.S. Department of Defense","University of Wisconsin-Madison","NCI NIH HHS","National Institutes of Health","National Science Foundation"],"pagination":["1139-1149"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7990711"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(6)"],"pubmed_abstract":["Melanoma evolution is a complex process. The role epidermal keratinocytes and dermal fibroblasts play in this process and the mechanisms involved in tumor-stroma interactions remain poorly understood. Here, we used a microfluidic platform to evaluate the cross-talk between human primary melanoma cells, keratinocytes and dermal fibroblasts. The microfluidic device included multiple circular chambers separated by a series of narrow connection channels. The microdevice design allowed us to develop a new cell patterning method based on air-walls, removing the need for hydrogel barriers, porous membranes, or external equipment. Using this method, we co-cultured melanoma cells in the presence of keratinocytes and/or dermal fibroblasts. The results demonstrated that the presence of dermal fibrobl"],"journal":["Lab on a chip"],"pubmed_title":["Microfluidic model with air-walls reveals fibroblasts and keratinocytes modulate melanoma cell phenotype, migration, and metabolism."],"pmcid":["PMC7990711"],"funding_grant_id":["AAB7173","R01 CA185747","R01 CA211082","W81XWH-18-PRCRP-IASF (CA181014)","CBET-1642287","R01 CA164492","R01 CA205101","NIH P30 P30AR066524"],"pubmed_authors":["Setaluri V","Rehman S","Ayuso JM","Denecke KM","Skala MC","Humayun M","Sadangi S","Lares M","Beebe DJ"],"additional_accession":[]},"is_claimable":false,"name":"Microfluidic model with air-walls reveals fibroblasts and keratinocytes modulate melanoma cell phenotype, migration, and metabolism.","description":"Melanoma evolution is a complex process. The role epidermal keratinocytes and dermal fibroblasts play in this process and the mechanisms involved in tumor-stroma interactions remain poorly understood. Here, we used a microfluidic platform to evaluate the cross-talk between human primary melanoma cells, keratinocytes and dermal fibroblasts. The microfluidic device included multiple circular chambers separated by a series of narrow connection channels. The microdevice design allowed us to develop a new cell patterning method based on air-walls, removing the need for hydrogel barriers, porous membranes, or external equipment. Using this method, we co-cultured melanoma cells in the presence of keratinocytes and/or dermal fibroblasts. The results demonstrated that the presence of dermal fibrobl","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2026-06-04T04:32:43.643Z","creation":"2026-05-05T03:11:45.565Z"},"accession":"S-EPMC7990711","cross_references":{"pubmed":["33533390"],"doi":["10.1039/d0lc00988a"]}}