{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin Y"],"funding":["Ministry of Education","H2020 Marie Sklodowska-Curie Actions","FP7 People: Marie-Curie Actions","Medical Research Council","Wellcome Trust","Australian Research Council","Engineering and Physical Sciences Research Council"],"pagination":["4034-4044"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7992134"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(3)"],"pubmed_abstract":["The evolution of life on earth eventually leads to the emergence of species with increased complexity and diversity. Similarly, evolutionary chemical space exploration in the laboratory is a key step to pursue the structural and functional diversity of supramolecular systems. Here, we present a powerful tool that enables rapid peptide diversification and employ it to expand the chemical space for supramolecular functions. Central to this strategy is the exploitation of palladium-catalyzed Suzuki-Miyaura cross-coupling reactions to direct combinatorial synthesis of peptide arrays in microtiter plates under an open atmosphere. Taking advantage of this <i>in situ</i> library design, our results unambiguously deliver a fertile platform for creating a set of intriguing peptide functions including green fluorescent protein-like peptide emitters with chemically encoded emission colors, hierarchical self-assembly into nano-objects, and macroscopic hydrogels. This work also offers opportunities for quickly surveying the diversified peptide arrays and thereby identifying the structural factors that modulate peptide properties."],"journal":["ACS nano"],"pubmed_title":["High-Throughput Peptide Derivatization toward Supramolecular Diversification in Microtiter Plates."],"pmcid":["PMC7992134"],"funding_grant_id":["098411/Z/12/Z","DP170100511","EP/M020398/1","EP/K020641/1","275433","DP140101888","MR/R015651/1","660757"],"pubmed_authors":["Gelmi A","Pashuck ET","Penna M","Higgins SG","Wojciechowski JP","Spicer CD","Lin Y","Yarovsky I","Stevens MM","Kim N","Wang ST"],"additional_accession":[]},"is_claimable":false,"name":"High-Throughput Peptide Derivatization toward Supramolecular Diversification in Microtiter Plates.","description":"The evolution of life on earth eventually leads to the emergence of species with increased complexity and diversity. Similarly, evolutionary chemical space exploration in the laboratory is a key step to pursue the structural and functional diversity of supramolecular systems. Here, we present a powerful tool that enables rapid peptide diversification and employ it to expand the chemical space for supramolecular functions. Central to this strategy is the exploitation of palladium-catalyzed Suzuki-Miyaura cross-coupling reactions to direct combinatorial synthesis of peptide arrays in microtiter plates under an open atmosphere. Taking advantage of this <i>in situ</i> library design, our results unambiguously deliver a fertile platform for creating a set of intriguing peptide functions including green fluorescent protein-like peptide emitters with chemically encoded emission colors, hierarchical self-assembly into nano-objects, and macroscopic hydrogels. This work also offers opportunities for quickly surveying the diversified peptide arrays and thereby identifying the structural factors that modulate peptide properties.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-04T22:00:41.418Z","creation":"2025-04-04T22:00:41.418Z"},"accession":"S-EPMC7992134","cross_references":{"pubmed":["33587607"],"doi":["10.1021/acsnano.0c05423"]}}