biostudies-literatureTsamou MKaren Vrijens is postdoctoral fellow of the FWOEuropean Research CouncilFlemish Research Council373-388https://www.ebi.ac.uk/biostudies/studies/S-EPMC7993149biostudies-literatureUnknown16(4)There is an increasing interest in microRNAs (miRNAs) as they are of utmost importance in gene regulation at the posttranscriptional level. Sex-related susceptibility for non-communicable diseases later in life could originate in early life. Until now, no data on sex-specific miRNA expression are available for the placenta. Therefore, we investigated the difference by sex of newborn's miRNA expression in human placental tissue. Within the ENVIR<i>ON</i>AGE birth cohort, miRNA and mRNA expression profiling was performed in 60 placentae (50% boys) using Agilent (8 × 60 K) microarrays. The distribution of chromosome locations was studied and pathway analysis of the identified sex-specific miRNAs in the placenta was carried out. Of the total 2558 miRNAs on the array, 597 miRNAs were expressed EpigeneticsGenome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIR<i>ON</i>AGE birth cohort study.PMC7993149ERC-2012-StG 310898310898FWO G073315N12D7718NMadhloum Nde Kok TMWinckelmans EWang CTsamou MNawrot TSNeven KYVrijens KfalseGenome-wide microRNA expression analysis in human placenta reveals sex-specific patterns: an ENVIR<i>ON</i>AGE birth cohort study.There is an increasing interest in microRNAs (miRNAs) as they are of utmost importance in gene regulation at the posttranscriptional level. Sex-related susceptibility for non-communicable diseases later in life could originate in early life. Until now, no data on sex-specific miRNA expression are available for the placenta. Therefore, we investigated the difference by sex of newborn's miRNA expression in human placental tissue. Within the ENVIR<i>ON</i>AGE birth cohort, miRNA and mRNA expression profiling was performed in 60 placentae (50% boys) using Agilent (8 × 60 K) microarrays. The distribution of chromosome locations was studied and pathway analysis of the identified sex-specific miRNAs in the placenta was carried out. Of the total 2558 miRNAs on the array, 597 miRNAs were expressed 2021-01-01T00:00:00Z2021 Apr2026-05-09T07:56:06.259Z2022-02-09T17:35:31.933ZS-EPMC79931493289269510.1080/15592294.2020.1803467