biostudies-literatureWeiss SLNICHD NIH HHSNIMH NIH HHSNINDS NIH HHSNIH HHSNIGMS NIH HHSe0360https://www.ebi.ac.uk/biostudies/studies/S-EPMC7994045biostudies-literatureUnknown3(3)<h4>Objectives</h4>The intestinal microbiome can modulate immune function through production of microbial-derived short-chain fatty acids. We explored whether intestinal dysbiosis in children with sepsis leads to changes in microbial-derived short-chain fatty acids in plasma and stool that are associated with immunometabolic dysfunction in peripheral blood mononuclear cells.<h4>Design</h4>Prospective observational pilot study.<h4>Setting</h4>Single academic PICU.<h4>Patients</h4>Forty-three children with sepsis/septic shock and 44 healthy controls.<h4>Measurements and main results</h4>Stool and plasma samples were serially collected for sepsis patients; stool was collected once for controls. The intestinal microbiome was assessed using 16S ribosomal RNA sequencing and alpha- and beta-diverCritical care explorationsDecreased Intestinal Microbiome Diversity in Pediatric Sepsis: A Conceptual Framework for Intestinal Dysbiosis to Influence Immunometabolic Function.PMC7994045R01 MH108592K23 GM110496K12 HD047349R01 OD010944R01 NS021328Wu GDMattei LMBushman FDZhang DMcGowan FXBalamuth FCollman RGBittinger KWallace DCFriedman ESGraham KWeiss SLBaldassano RNBush JLee JJfalseDecreased Intestinal Microbiome Diversity in Pediatric Sepsis: A Conceptual Framework for Intestinal Dysbiosis to Influence Immunometabolic Function.<h4>Objectives</h4>The intestinal microbiome can modulate immune function through production of microbial-derived short-chain fatty acids. We explored whether intestinal dysbiosis in children with sepsis leads to changes in microbial-derived short-chain fatty acids in plasma and stool that are associated with immunometabolic dysfunction in peripheral blood mononuclear cells.<h4>Design</h4>Prospective observational pilot study.<h4>Setting</h4>Single academic PICU.<h4>Patients</h4>Forty-three children with sepsis/septic shock and 44 healthy controls.<h4>Measurements and main results</h4>Stool and plasma samples were serially collected for sepsis patients; stool was collected once for controls. The intestinal microbiome was assessed using 16S ribosomal RNA sequencing and alpha- and beta-diver2021-01-01T00:00:00Z2021 Mar2026-03-18T13:43:48.429Z2025-04-04T11:43:48.101ZS-EPMC79940453378643610.1097/CCE.0000000000000360