{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lambrecht E"],"funding":["FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu","Bijzonder Onderzoeksfonds UGent"],"pagination":["192"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7997361"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(3)"],"pubmed_abstract":["Along with (in) direct contact with animals and a contaminated environment, humans are exposed to antibiotic-resistant bacteria by consumption of food. The implications of ingesting antibiotic-resistant commensal bacteria are unknown, as dose-response data on resistance transfer and spreading in our gut is lacking. In this study, transfer of a resistance plasmid (IncF), harbouring several antibiotic resistance genes, from a commensal <i>E. coli</i> strain towards human intestinal microbiota was assessed using a Mucosal Simulator of the Human Intestinal Ecosystem (M-SHIME). More specifically, the effect of the initial <i>E. coli</i> plasmid donor concentration (10<sup>5</sup> and 10<sup>7</sup> CFU/meal), antibiotic treatment (cefotaxime) and human individual (n = 6) on plasmid transfer tow"],"journal":["Life (Basel, Switzerland)"],"pubmed_title":["Transfer of Antibiotic Resistance Plasmid from Commensal <i>E. coli</i> Towards Human Intestinal Microbiota in the M-SHIME: Effect of <i>E. coli</i> dosis, Human Individual and Antibiotic Use."],"pmcid":["PMC7997361"],"funding_grant_id":["BOF17/GOA/032","RF 13/9 TRAMRISK"],"pubmed_authors":["Lambrecht E","Wiele TV","Heyndrickx M","Coillie EV","Boon N"],"additional_accession":[]},"is_claimable":false,"name":"Transfer of Antibiotic Resistance Plasmid from Commensal <i>E. coli</i> Towards Human Intestinal Microbiota in the M-SHIME: Effect of <i>E. coli</i> dosis, Human Individual and Antibiotic Use.","description":"Along with (in) direct contact with animals and a contaminated environment, humans are exposed to antibiotic-resistant bacteria by consumption of food. The implications of ingesting antibiotic-resistant commensal bacteria are unknown, as dose-response data on resistance transfer and spreading in our gut is lacking. In this study, transfer of a resistance plasmid (IncF), harbouring several antibiotic resistance genes, from a commensal <i>E. coli</i> strain towards human intestinal microbiota was assessed using a Mucosal Simulator of the Human Intestinal Ecosystem (M-SHIME). More specifically, the effect of the initial <i>E. coli</i> plasmid donor concentration (10<sup>5</sup> and 10<sup>7</sup> CFU/meal), antibiotic treatment (cefotaxime) and human individual (n = 6) on plasmid transfer tow","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-19T17:03:59.262Z","creation":"2022-02-09T14:47:42.422Z"},"accession":"S-EPMC7997361","cross_references":{"pubmed":["33670965"],"doi":["10.3390/life11030192"]}}