biostudies-literatureLambrecht EFOD Volksgezondheid, Veiligheid van de Voedselketen en LeefmilieuBijzonder Onderzoeksfonds UGent192https://www.ebi.ac.uk/biostudies/studies/S-EPMC7997361biostudies-literatureUnknown11(3)Along with (in) direct contact with animals and a contaminated environment, humans are exposed to antibiotic-resistant bacteria by consumption of food. The implications of ingesting antibiotic-resistant commensal bacteria are unknown, as dose-response data on resistance transfer and spreading in our gut is lacking. In this study, transfer of a resistance plasmid (IncF), harbouring several antibiotic resistance genes, from a commensal <i>E. coli</i> strain towards human intestinal microbiota was assessed using a Mucosal Simulator of the Human Intestinal Ecosystem (M-SHIME). More specifically, the effect of the initial <i>E. coli</i> plasmid donor concentration (10⁵ and 10⁷ CFU/meal), antibiotic treatment (cefotaxime) and human individual (n = 6) on plasmid transfer towLife (Basel, Switzerland)Transfer of Antibiotic Resistance Plasmid from Commensal <i>E. coli</i> Towards Human Intestinal Microbiota in the M-SHIME: Effect of <i>E. coli</i> dosis, Human Individual and Antibiotic Use.PMC7997361BOF17/GOA/032RF 13/9 TRAMRISKLambrecht EWiele TVHeyndrickx MCoillie EVBoon NfalseTransfer of Antibiotic Resistance Plasmid from Commensal <i>E. coli</i> Towards Human Intestinal Microbiota in the M-SHIME: Effect of <i>E. coli</i> dosis, Human Individual and Antibiotic Use.Along with (in) direct contact with animals and a contaminated environment, humans are exposed to antibiotic-resistant bacteria by consumption of food. The implications of ingesting antibiotic-resistant commensal bacteria are unknown, as dose-response data on resistance transfer and spreading in our gut is lacking. In this study, transfer of a resistance plasmid (IncF), harbouring several antibiotic resistance genes, from a commensal <i>E. coli</i> strain towards human intestinal microbiota was assessed using a Mucosal Simulator of the Human Intestinal Ecosystem (M-SHIME). More specifically, the effect of the initial <i>E. coli</i> plasmid donor concentration (10⁵ and 10⁷ CFU/meal), antibiotic treatment (cefotaxime) and human individual (n = 6) on plasmid transfer tow2021-01-01T00:00:00Z2021 Feb2025-04-19T17:03:59.262Z2022-02-09T14:47:42.422ZS-EPMC79973613367096510.3390/life11030192