biostudies-literatureOungsakul Pthe John &amp; Mary Kibble FoundationUniversity of Queensland Collaboration and Industry Engagement FundThe Australian Animal Cancer Foundationthe John & Mary Kibble Foundation38https://www.ebi.ac.uk/biostudies/studies/S-EPMC7997418biostudies-literatureUnknown8(3)Visceral hemangiosarcoma (HSA) is one of the more frequent cancers in dogs and has a high metastatic rate and poor prognosis, as clinical signs only become apparent in advanced stages of tumor development. In order to improve early and differential diagnostic capabilities and hence, prognosis for dogs with HSA, two types of biomarker are needed: a point-of-care diagnostic biomarker and a prognostic biomarker-preferentially based on samples obtained with minimally invasive methods. In this study, we applied a lectin magnetic bead array-coupled tandem mass spectrometry (LeMBA-MS/MS) workflow through discovery and validation phases to discover serum glycoprotein biomarker candidates for canine HSA. By this approach, we found that <i>Datura stramonium</i> (DSA), wheat germ agglutinin (WGA), <iVeterinary sciencesCandidate Glycoprotein Biomarkers for Canine Visceral Hemangiosarcoma and Validation Using Semi-Quantitative Lectin/Immunohistochemical Assays.PMC7997418not applicableDuffy DO'Leary COungsakul PBielefeldt-Ohmann HChoi EMohamed AShah AKHill MMfalseCandidate Glycoprotein Biomarkers for Canine Visceral Hemangiosarcoma and Validation Using Semi-Quantitative Lectin/Immunohistochemical Assays.Visceral hemangiosarcoma (HSA) is one of the more frequent cancers in dogs and has a high metastatic rate and poor prognosis, as clinical signs only become apparent in advanced stages of tumor development. In order to improve early and differential diagnostic capabilities and hence, prognosis for dogs with HSA, two types of biomarker are needed: a point-of-care diagnostic biomarker and a prognostic biomarker-preferentially based on samples obtained with minimally invasive methods. In this study, we applied a lectin magnetic bead array-coupled tandem mass spectrometry (LeMBA-MS/MS) workflow through discovery and validation phases to discover serum glycoprotein biomarker candidates for canine HSA. By this approach, we found that <i>Datura stramonium</i> (DSA), wheat germ agglutinin (WGA), <i2021-01-01T00:00:00Z2021 Feb2026-04-07T23:26:09.879Z2022-02-09T14:48:29.923ZS-EPMC79974183367350710.3390/vetsci8030038