{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9"],"submitter":["Sadaqat B"],"pubmed_abstract":["<i>Thermotoga maritima</i> (<i>Tma</i>) contains genes encoding various hyperthermophilic enzymes with great potential for industrial applications. The gene TM1752 in <i>Tma</i> genome has been annotated as cellulase gene encoding protein Cel5B. In this work, the gene TM1752 was cloned and expressed in <i>Escherichia coli</i>, and the recombinant enzyme was purified and characterized. Interestingly, the purified enzyme exhibited specific activities of 416 and 215 U/mg on substrates galactomannan and carboxy methyl cellulose, which is the highest among thermophilic mannanases. However, the putative enzyme did not show sequence homology with any of the previously reported mannanases; therefore, the enzyme Cel5B was identified as bifunctional mannanase and cellulase and renamed as Man/Cel5B. "],"journal":["Frontiers in bioengineering and biotechnology"],"pagination":["637649"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8007966"],"repository":["biostudies-literature"],"pubmed_title":["Man/Cel5B, a Bifunctional Enzyme Having the Highest Mannanase Activity in the Hyperthermic Environment."],"pmcid":["PMC8007966"],"pubmed_authors":["Zuo W","Shao W","Rupani PF","Sadaqat B","Wang H","Sha C"],"additional_accession":[]},"is_claimable":false,"name":"Man/Cel5B, a Bifunctional Enzyme Having the Highest Mannanase Activity in the Hyperthermic Environment.","description":"<i>Thermotoga maritima</i> (<i>Tma</i>) contains genes encoding various hyperthermophilic enzymes with great potential for industrial applications. The gene TM1752 in <i>Tma</i> genome has been annotated as cellulase gene encoding protein Cel5B. In this work, the gene TM1752 was cloned and expressed in <i>Escherichia coli</i>, and the recombinant enzyme was purified and characterized. Interestingly, the purified enzyme exhibited specific activities of 416 and 215 U/mg on substrates galactomannan and carboxy methyl cellulose, which is the highest among thermophilic mannanases. However, the putative enzyme did not show sequence homology with any of the previously reported mannanases; therefore, the enzyme Cel5B was identified as bifunctional mannanase and cellulase and renamed as Man/Cel5B. ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-04-03T21:04:45.233Z","creation":"2022-02-09T13:37:43.495Z"},"accession":"S-EPMC8007966","cross_references":{"pubmed":["33796509"],"doi":["10.3389/fbioe.2021.637649"]}}