biostudies-literatureTang Lthe Natural Science Foundation of Shanxi ProvinceSoutheast Asia Biodiversity Research Institute, Chinese Academy of Sciencesthe National Natural Science Foundation of China1253-1255https://www.ebi.ac.uk/biostudies/studies/S-EPMC8008875biostudies-literatureUnknown6(3)<i>Phaius hainanensis</i> C. Z. Tang et S. J. Cheng is a species with extremely small populations and is endemic to China. Genetic data of this orchid species is minimal. With the aim to identify appropriate chloroplast markers for the use in conservation biology studies, the plastome of <i>P. hainanenisis</i> was assembled. The plastome of <i>P. hainanensis</i> is 158,314 bp in length and contains a large single copy region of 86,700 bp in length, a small single copy region of 18,452 bp, and a pair of inverted repeats of 26,581 bp. The annotation predicted 114 unique genes, including 80 protein-coding, 30 <i>tRNA</i>s, and four <i>rRNA</i>s. Seventeen genes contained a single intron and two genes (<i>clpP</i> and <i>ycf3</i>) have two introns. The GC content of <i>P. hainanensis</i> is 36.9%. Phylogenetic analysis indicated <i>P. hainanensis</i> is closely related to <i>P. tancarvilleae,</i> and it also supported that <i>Phaius</i> and <i>Calanthe</i> are sister groups. The plastome data reported in this study will contribute to further studies of phylogeny and conservation of <i>Phaius</i> species.Mitochondrial DNA. Part B, ResourcesThe plastome of <i>Phaius hainanensis</i> (Orchidaceae): an endangered species endemic to Hainan province, China.PMC800887531870183201901D111223Y4ZK111B01Ge LPTang LLuo YTang HQfalseThe plastome of <i>Phaius hainanensis</i> (Orchidaceae): an endangered species endemic to Hainan province, China.<i>Phaius hainanensis</i> C. Z. Tang et S. J. Cheng is a species with extremely small populations and is endemic to China. Genetic data of this orchid species is minimal. With the aim to identify appropriate chloroplast markers for the use in conservation biology studies, the plastome of <i>P. hainanenisis</i> was assembled. The plastome of <i>P. hainanensis</i> is 158,314 bp in length and contains a large single copy region of 86,700 bp in length, a small single copy region of 18,452 bp, and a pair of inverted repeats of 26,581 bp. The annotation predicted 114 unique genes, including 80 protein-coding, 30 <i>tRNA</i>s, and four <i>rRNA</i>s. Seventeen genes contained a single intron and two genes (<i>clpP</i> and <i>ycf3</i>) have two introns. The GC content of <i>P. hainanensis</i> is 36.9%. Phylogenetic analysis indicated <i>P. hainanensis</i> is closely related to <i>P. tancarvilleae,</i> and it also supported that <i>Phaius</i> and <i>Calanthe</i> are sister groups. The plastome data reported in this study will contribute to further studies of phylogeny and conservation of <i>Phaius</i> species.2021-01-01T00:00:00Z2021 Mar2024-10-15T22:49:40.557Z2022-02-10T09:23:34.361ZS-EPMC80088753382910010.1080/23802359.2021.1904801