biostudies-literatureUnknown8Cappele JConjugative transfer is a major threat to global health since it contributes to the spread of antibiotic resistance genes and virulence factors among commensal and pathogenic bacteria. To allow their transfer, mobile genetic elements including Integrative and Conjugative Elements (ICEs) use a specialized conjugative apparatus related to Type IV secretion systems (Conj-T4SS). Therefore, Conj-T4SSs are excellent targets for strategies that aim to limit the spread of antibiotic resistance. In this study, we combined structural, biochemical and biophysical approaches to study OrfG, a protein that belongs to Conj-T4SS of ICE<i>St3</i> from <i>Streptococcus thermophilus</i>. Structural analysis of OrfG by X-ray crystallography revealed that OrfG central domain is similar to VirB8-like proteins bFrontiers in molecular biosciences642606https://www.ebi.ac.uk/biostudies/studies/S-EPMC8012802biostudies-literatureStructural and Biochemical Analysis of OrfG: The VirB8-like Component of the Conjugative Type IV Secretion System of ICE<i>St3</i> From <i>Streptococcus thermophilus</i>.PMC8012802Favier FDouzi BDhalleine TPayot SSavko MMohamad Ali AMathiot SDidierjean CLeblond-Bourget NCappele JfalseStructural and Biochemical Analysis of OrfG: The VirB8-like Component of the Conjugative Type IV Secretion System of ICE<i>St3</i> From <i>Streptococcus thermophilus</i>.Conjugative transfer is a major threat to global health since it contributes to the spread of antibiotic resistance genes and virulence factors among commensal and pathogenic bacteria. To allow their transfer, mobile genetic elements including Integrative and Conjugative Elements (ICEs) use a specialized conjugative apparatus related to Type IV secretion systems (Conj-T4SS). Therefore, Conj-T4SSs are excellent targets for strategies that aim to limit the spread of antibiotic resistance. In this study, we combined structural, biochemical and biophysical approaches to study OrfG, a protein that belongs to Conj-T4SS of ICE<i>St3</i> from <i>Streptococcus thermophilus</i>. Structural analysis of OrfG by X-ray crystallography revealed that OrfG central domain is similar to VirB8-like proteins b2021-01-01T00:00:00Z20212026-04-16T11:40:38.224Z2022-02-10T15:44:38.482ZS-EPMC80128023381655710.3389/fmolb.2021.642606