biostudies-literaturePandya CDNCATS NIH HHSUniversity of KentuckyNINDS NIH HHSNational Institutes of Health13-23https://www.ebi.ac.uk/biostudies/studies/S-EPMC8016702biostudies-literatureUnknown231Oligodendrocyte progenitor cells (OPCs) in the infant brain give rise to mature oligodendrocytes that myelinate CNS axons. OPCs are particularly vulnerable to oxidative stress that occurs in many forms of brain injury. One common cause of infant brain injury is neonatal intraventricular hemorrhage (IVH), which releases blood into the CSF and brain parenchyma of preterm infants. Although blood contains the powerful oxidant hemoglobin, the direct effects of hemoglobin on OPCs have not been studied. We utilized a cell culture system to test if hemoglobin induced free radical production and mitochondrial dysfunction in OPCs. We also tested if phenelzine (PLZ), an FDA-approved antioxidant drug, could protect OPCs from hemoglobin-induced oxidative stress. OPCs were isolated from Sprague Dawley rTranslational research : the journal of laboratory and clinical medicineHemoglobin induces oxidative stress and mitochondrial dysfunction in oligodendrocyte progenitor cells.PMC8016702UL1 TR001998KL2 TR001996K08 NS112580Vekaria HGensel JCMiller BASlone SAJoseph BSullivan PGPandya CDfalseHemoglobin induces oxidative stress and mitochondrial dysfunction in oligodendrocyte progenitor cells.Oligodendrocyte progenitor cells (OPCs) in the infant brain give rise to mature oligodendrocytes that myelinate CNS axons. OPCs are particularly vulnerable to oxidative stress that occurs in many forms of brain injury. One common cause of infant brain injury is neonatal intraventricular hemorrhage (IVH), which releases blood into the CSF and brain parenchyma of preterm infants. Although blood contains the powerful oxidant hemoglobin, the direct effects of hemoglobin on OPCs have not been studied. We utilized a cell culture system to test if hemoglobin induced free radical production and mitochondrial dysfunction in OPCs. We also tested if phenelzine (PLZ), an FDA-approved antioxidant drug, could protect OPCs from hemoglobin-induced oxidative stress. OPCs were isolated from Sprague Dawley r2021-01-01T00:00:00Z2021 May2025-04-04T10:15:51.726Z2025-04-04T10:15:51.726ZS-EPMC80167023346082410.1016/j.trsl.2021.01.005