biostudies-literatureTavares-Ferreira DNational Institute of Neurological Disorders and StrokeNINDS NIH HHS129-140https://www.ebi.ac.uk/biostudies/studies/S-EPMC8019688biostudies-literatureUnknown91(1)<h4>Background</h4>There are clinically relevant sex differences in acute and chronic pain mechanisms, but we are only beginning to understand their mechanistic basis. Transcriptome analyses of rodent whole dorsal root ganglion (DRG) have revealed sex differences, mostly in immune cells. We examined the transcriptome and translatome of the mouse DRG with the goal of identifying sex differences.<h4>Methods</h4>We used translating ribosome affinity purification sequencing and behavioral pharmacology to test the hypothesis that in Nav1.8-positive neurons, most of which are nociceptors, translatomes would differ by sex.<h4>Results</h4>We found 80 genes with sex differential expression in the whole DRG transcriptome and 66 genes whose messenger RNAs were sex differentially actively translated (Biological psychiatrySex Differences in Nociceptor Translatomes Contribute to Divergent Prostaglandin Signaling in Male and Female Mice.PMC8019688R01 NS065926R01 NS102161Akopian ANBarragan-Iglesias PTavares-Ferreira DRay PRSankaranarayanan IDussor GMegat SWangzhou AUttarkar RMejia GLShiers SPrice TJfalseSex Differences in Nociceptor Translatomes Contribute to Divergent Prostaglandin Signaling in Male and Female Mice.<h4>Background</h4>There are clinically relevant sex differences in acute and chronic pain mechanisms, but we are only beginning to understand their mechanistic basis. Transcriptome analyses of rodent whole dorsal root ganglion (DRG) have revealed sex differences, mostly in immune cells. We examined the transcriptome and translatome of the mouse DRG with the goal of identifying sex differences.<h4>Methods</h4>We used translating ribosome affinity purification sequencing and behavioral pharmacology to test the hypothesis that in Nav1.8-positive neurons, most of which are nociceptors, translatomes would differ by sex.<h4>Results</h4>We found 80 genes with sex differential expression in the whole DRG transcriptome and 66 genes whose messenger RNAs were sex differentially actively translated (2022-01-01T00:00:00Z2022 Jan2026-05-02T11:19:23.318Z2025-02-18T22:34:28.966ZS-EPMC80196883330901610.1016/j.biopsych.2020.09.022