{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["van Erp S"],"funding":["EMBO","Medical Research Council","NINDS NIH HHS","Seventh Framework Programme","Sight Research UK","National Institutes of Health","Wellcome Trust","Multiple Sclerosis Society"],"pagination":["113594"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8024785"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["339"],"pubmed_abstract":["Regeneration capacity is reduced as CNS axons mature. Using laser-mediated axotomy, proteomics and puromycin-based tagging of newly-synthesized proteins in a human embryonic stem cell-derived neuron culture system that allows isolation of axons from cell bodies, we show here that efficient regeneration in younger axons (d45 in culture) is associated with local axonal protein synthesis (local translation). Enhanced regeneration, promoted by co-culture with human glial precursor cells, is associated with increased axonal synthesis of proteins, including those constituting the translation machinery itself. Reduced regeneration, as occurs with the maturation of these axons by d65 in culture, correlates with reduced levels of axonal proteins involved in translation and an inability to respond b"],"journal":["Experimental neurology"],"pubmed_title":["Age-related loss of axonal regeneration is reflected by the level of local translation."],"pmcid":["PMC8024785"],"funding_grant_id":["MR/R004544/1","79","SAC 026","208402/Z/17/Z","MR/R004463/1","104783/Z/14/Z","G0300336","R01 NS117821","MR/V002694/1"],"pubmed_authors":["van Berkel AA","Eva R","Twiss JL","Sahoo PK","Feenstra EM","Fawcett JW","Wagstaff LJ","Ffrench-Constant C","van Erp S"],"additional_accession":[]},"is_claimable":false,"name":"Age-related loss of axonal regeneration is reflected by the level of local translation.","description":"Regeneration capacity is reduced as CNS axons mature. Using laser-mediated axotomy, proteomics and puromycin-based tagging of newly-synthesized proteins in a human embryonic stem cell-derived neuron culture system that allows isolation of axons from cell bodies, we show here that efficient regeneration in younger axons (d45 in culture) is associated with local axonal protein synthesis (local translation). Enhanced regeneration, promoted by co-culture with human glial precursor cells, is associated with increased axonal synthesis of proteins, including those constituting the translation machinery itself. Reduced regeneration, as occurs with the maturation of these axons by d65 in culture, correlates with reduced levels of axonal proteins involved in translation and an inability to respond b","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-01T06:05:15.913Z","creation":"2025-06-01T01:35:22.808Z"},"accession":"S-EPMC8024785","cross_references":{"pubmed":["33450233"],"doi":["10.1016/j.expneurol.2020.113594"]}}