{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Howden SE"],"funding":["NIDDK NIH HHS","Nierstichting","National Institutes of Health","Chan Zuckerberg Initiative","NIGMS NIH HHS","Australian Research Council"],"pagination":["671-684.e6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8026527"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(4)"],"pubmed_abstract":["During development, distinct progenitors contribute to the nephrons versus the ureteric epithelium of the kidney. Indeed, previous human pluripotent stem-cell-derived models of kidney tissue either contain nephrons or pattern specifically to the ureteric epithelium. By re-analyzing the transcriptional distinction between distal nephron and ureteric epithelium in human fetal kidney, we show here that, while existing nephron-containing kidney organoids contain distal nephron epithelium and no ureteric epithelium, this distal nephron segment alone displays significant in vitro plasticity and can adopt a ureteric epithelial tip identity when isolated and cultured in defined conditions. \"Induced\" ureteric epithelium cultures can be cryopreserved, serially passaged without loss of identity, and transitioned toward a collecting duct fate. Cultures harboring loss-of-function mutations in PKHD1 also recapitulate the cystic phenotype associated with autosomal recessive polycystic kidney disease."],"journal":["Cell stem cell"],"pubmed_title":["Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk."],"pmcid":["PMC8026527"],"funding_grant_id":["T32 GM145304","UH3 DK107344","U01 DK107350"],"pubmed_authors":["Jain S","Groenewegen E","Little MH","Starks L","Tan KS","Howden SE","Forbes TA","Chen YH","Holloway EM","Wilson SB","Vanslambrouck JM","Spence JR"],"additional_accession":[]},"is_claimable":false,"name":"Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk.","description":"During development, distinct progenitors contribute to the nephrons versus the ureteric epithelium of the kidney. Indeed, previous human pluripotent stem-cell-derived models of kidney tissue either contain nephrons or pattern specifically to the ureteric epithelium. By re-analyzing the transcriptional distinction between distal nephron and ureteric epithelium in human fetal kidney, we show here that, while existing nephron-containing kidney organoids contain distal nephron epithelium and no ureteric epithelium, this distal nephron segment alone displays significant in vitro plasticity and can adopt a ureteric epithelial tip identity when isolated and cultured in defined conditions. \"Induced\" ureteric epithelium cultures can be cryopreserved, serially passaged without loss of identity, and transitioned toward a collecting duct fate. Cultures harboring loss-of-function mutations in PKHD1 also recapitulate the cystic phenotype associated with autosomal recessive polycystic kidney disease.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-05-02T22:39:53.603Z","creation":"2025-04-05T09:58:39.583Z"},"accession":"S-EPMC8026527","cross_references":{"pubmed":["33378647"],"doi":["10.1016/j.stem.2020.12.001"]}}