biostudies-literatureOnimaru HJapan Society for the Promotion of Science645904https://www.ebi.ac.uk/biostudies/studies/S-EPMC8027497biostudies-literatureUnknown12It is supposed that the nucleus of the solitary tract (NTS) in the dorsal medulla includes gas sensor cells responsive to hypercapnia or hypoxia in the central nervous system. In the present study, we analyzed cellular responses to hypercapnia and hypoxia in the NTS region of newborn rat <i>in vitro</i> preparation. The brainstem and spinal cord were isolated from newborn rat (P0-P4) and were transversely cut at the level of the rostral area postrema. To detect cellular responses, calcium indicator Oregon Green was pressure-injected into the NTS just beneath the cut surface of either the caudal or rostral block of the medulla, and the preparation was superfused with artificial cerebrospinal fluid (25-26°C). We examined cellular responses initially to hypercapnic stimulation (to 8% CO<sub>2Frontiers in physiologyCalcium Imaging Analysis of Cellular Responses to Hypercapnia and Hypoxia in the NTS of Newborn Rat Brainstem Preparation.PMC802749719K06946Yazawa IOnimaru HFukushi IOkada YTakeda KfalseCalcium Imaging Analysis of Cellular Responses to Hypercapnia and Hypoxia in the NTS of Newborn Rat Brainstem Preparation.It is supposed that the nucleus of the solitary tract (NTS) in the dorsal medulla includes gas sensor cells responsive to hypercapnia or hypoxia in the central nervous system. In the present study, we analyzed cellular responses to hypercapnia and hypoxia in the NTS region of newborn rat <i>in vitro</i> preparation. The brainstem and spinal cord were isolated from newborn rat (P0-P4) and were transversely cut at the level of the rostral area postrema. To detect cellular responses, calcium indicator Oregon Green was pressure-injected into the NTS just beneath the cut surface of either the caudal or rostral block of the medulla, and the preparation was superfused with artificial cerebrospinal fluid (25-26°C). We examined cellular responses initially to hypercapnic stimulation (to 8% CO<sub>22021-01-01T00:00:00Z20212026-05-08T03:01:37.444Z2025-05-18T11:17:14.768ZS-EPMC80274973384118210.3389/fphys.2021.645904