biostudies-literatureHuang JNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (National Science Foundation of China)1174-1192https://www.ebi.ac.uk/biostudies/studies/S-EPMC8027887biostudies-literatureUnknown28(4)Hepatic ischemic reperfusion injury (IRI) is a common complication of liver surgery. Although an imbalance between mitochondrial fission and fusion has been identified as the cause of IRI, the detailed mechanism remains unclear. Augmenter of liver regeneration (ALR) was reported to prevent mitochondrial fission by inhibiting dynamin-related protein 1 (Drp1) phosphorylation, contributing partially to its liver protection. Apart from phosphorylation, Drp1 activity is also regulated by small ubiquitin-like modification (SUMOylation), which accelerates mitochondrial fission. This study aimed to investigate whether ALR-mediated protection from hepatic IRI might be associated with an effect on Drp1 SUMOylation. Liver tissues were harvested from both humans and from heterozygous ALR knockout miceCell death and differentiationInhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.PMC802788731771279Dong YHuang JAn WXie PfalseInhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.Hepatic ischemic reperfusion injury (IRI) is a common complication of liver surgery. Although an imbalance between mitochondrial fission and fusion has been identified as the cause of IRI, the detailed mechanism remains unclear. Augmenter of liver regeneration (ALR) was reported to prevent mitochondrial fission by inhibiting dynamin-related protein 1 (Drp1) phosphorylation, contributing partially to its liver protection. Apart from phosphorylation, Drp1 activity is also regulated by small ubiquitin-like modification (SUMOylation), which accelerates mitochondrial fission. This study aimed to investigate whether ALR-mediated protection from hepatic IRI might be associated with an effect on Drp1 SUMOylation. Liver tissues were harvested from both humans and from heterozygous ALR knockout mice2021-01-01T00:00:00Z2021 Apr2025-04-25T22:29:33.414Z2025-04-06T09:03:35.304ZS-EPMC80278873311021610.1038/s41418-020-00641-7