biostudies-literatureUnknown9(5)Yang L<h4>Background</h4>Ventricular-like human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) exhibit the electrophysiological characteristics of spontaneous beating. Previous studies demonstrated that dexmedetomidine (DMED), a highly selective and widely used α₂-adrenoceptor agonist for sedation, analgesia, and stress management, may induce antiarrhythmic effects, especially ventricular tachycardia. However, the underlying mechanisms of the DMED-mediated antiarrhythmic effects remain to be fully elucidated.<h4>Methods</h4>A conventional patch-clamp recording method was used to investigate the direct effects of DMED on spontaneous action potentials, pacemaker currents (<i>I</i> _f), potassium (K⁺) channel currents (<i>I</i> _K1 and <i>Annals of translational medicine399https://www.ebi.ac.uk/biostudies/studies/S-EPMC8033317biostudies-literatureDexmedetomidine exhibits antiarrhythmic effects on human-induced pluripotent stem cell-derived cardiomyocytes through a Na/Ca channel-mediated mechanism.PMC8033317Zheng JWitman NWu LYang LZhang JTan YFu WWang WGong YfalseDexmedetomidine exhibits antiarrhythmic effects on human-induced pluripotent stem cell-derived cardiomyocytes through a Na/Ca channel-mediated mechanism.<h4>Background</h4>Ventricular-like human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) exhibit the electrophysiological characteristics of spontaneous beating. Previous studies demonstrated that dexmedetomidine (DMED), a highly selective and widely used α₂-adrenoceptor agonist for sedation, analgesia, and stress management, may induce antiarrhythmic effects, especially ventricular tachycardia. However, the underlying mechanisms of the DMED-mediated antiarrhythmic effects remain to be fully elucidated.<h4>Methods</h4>A conventional patch-clamp recording method was used to investigate the direct effects of DMED on spontaneous action potentials, pacemaker currents (<i>I</i> _f), potassium (K⁺) channel currents (<i>I</i> _K1 and <i>2021-01-01T00:00:00Z2021 Mar2025-04-04T21:24:34.013Z2025-04-04T21:24:34.013ZS-EPMC80333173384262010.21037/atm-20-5898