biostudies-literatureGerberich BGOak Ridge Institute for Science and EducationNEI NIH HHSRRD VANational Institutes of HealthUS Department of Veterans Affairs120735https://www.ebi.ac.uk/biostudies/studies/S-EPMC8044034biostudies-literatureUnknown271The central vision-threatening event in glaucoma is dysfunction and loss of retinal ganglion cells (RGCs), thought to be promoted by local tissue deformations. Here, we sought to reduce tissue deformation near the optic nerve head by selectively stiffening the peripapillary sclera, i.e. the scleral region immediately adjacent to the optic nerve head. Previous scleral stiffening studies to treat glaucoma or myopia have used either pan-scleral stiffening (not regionally selective) or regionally selective stiffening with limited access to the posterior globe. We present a method for selectively stiffening the peripapillary sclera using a transpupillary annular light beam to activate methylene blue administered by retrobulbar injection. Unlike prior approaches to photocrosslinking in the eye, BiomaterialsTranspupillary collagen photocrosslinking for targeted modulation of ocular biomechanics.PMC8044034IK2 RX002342IK6 RX003134R01 EY025286Hejri AMacLeod NARead ATToothman MGEthier CRGerberich BGSridhar SGersch HGGupta SGoyal VPardue MTToporek AMHannon BGSchwaner SAGershon GSSanchez-Rodriguez GNichols LMFeola AJRitch MDPrausnitz MRWinger EJGrossniklaus HESchrader Echeverri EfalseTranspupillary collagen photocrosslinking for targeted modulation of ocular biomechanics.The central vision-threatening event in glaucoma is dysfunction and loss of retinal ganglion cells (RGCs), thought to be promoted by local tissue deformations. Here, we sought to reduce tissue deformation near the optic nerve head by selectively stiffening the peripapillary sclera, i.e. the scleral region immediately adjacent to the optic nerve head. Previous scleral stiffening studies to treat glaucoma or myopia have used either pan-scleral stiffening (not regionally selective) or regionally selective stiffening with limited access to the posterior globe. We present a method for selectively stiffening the peripapillary sclera using a transpupillary annular light beam to activate methylene blue administered by retrobulbar injection. Unlike prior approaches to photocrosslinking in the eye, 2021-01-01T00:00:00Z2021 Apr2025-04-29T11:34:48.802Z2025-04-06T19:54:54.003ZS-EPMC80440343372157110.1016/j.biomaterials.2021.120735