biostudies-literatureCichy ADeutsche ForschungsgemeinschaftNIDCD NIH HHSNIDCDNIH2687-2697.e4https://www.ebi.ac.uk/biostudies/studies/S-EPMC8049193biostudies-literatureUnknown29(16)The mammalian main olfactory pathway detects myriad volatile chemicals using >1,000 odorant receptor (OR) genes, which are organized into two phylogenetically distinct classes (class I and class II). An important question is how these evolutionarily conserved classes contribute to odor perception. Here, we report functional inactivation of a large number of class I ORs in mice via identification and deletion of a local cis-acting enhancer in the class I gene cluster. This manipulation reduced expression of half of the 131 intact class I genes. The resulting class I-depleted mice exhibited a significant reduction in the number of glomeruli responding to carboxylic acids-chemicals associated with microbial action and body odors. These mice also exhibit a change in odor perception marked by aCurrent biology : CBGenetic Depletion of Class I Odorant Receptors Impacts Perception of Carboxylic Acids.PMC8049193R01DC013576F32 DC000357R03 DC014565R01 DC013576R03DC014565CI 222/1-1Shah ACichy ABozza TDewan AKaye SfalseGenetic Depletion of Class I Odorant Receptors Impacts Perception of Carboxylic Acids.The mammalian main olfactory pathway detects myriad volatile chemicals using >1,000 odorant receptor (OR) genes, which are organized into two phylogenetically distinct classes (class I and class II). An important question is how these evolutionarily conserved classes contribute to odor perception. Here, we report functional inactivation of a large number of class I ORs in mice via identification and deletion of a local cis-acting enhancer in the class I gene cluster. This manipulation reduced expression of half of the 131 intact class I genes. The resulting class I-depleted mice exhibited a significant reduction in the number of glomeruli responding to carboxylic acids-chemicals associated with microbial action and body odors. These mice also exhibit a change in odor perception marked by a2019-01-01T00:00:00Z2019 Aug2025-04-29T10:19:51.855Z2022-02-09T15:56:17.724ZS-EPMC80491933137861110.1016/j.cub.2019.06.085