{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kiguchi Y"],"funding":["Japan Society for the Promotion of Science"],"pagination":["8201"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8050046"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targeting \"hot regions,\" i.e., amino acid substitutions therein frequently increase the affinities, is desirable for straightforward discovery of valuable mutants. We here report two \"designed\" site-directed mutagenesis (A and B) targeted the N-terminal 1-10 positions of the V<sub>H</sub> framework region 1 that successfully improved an anti-cortisol single-chain Fv fragment (K<sub>a</sub>, 3.6 × 10<sup>8</sup> M<sup>-1</sup>). Mutagenesis A substituted the amino acids at the position 1-3, 5-7, 9 and 10 with a limited set of substitutions to generate only 1,"],"journal":["Scientific reports"],"pubmed_title":["The V<sub>H</sub> framework region 1 as a target of efficient mutagenesis for generating a variety of affinity-matured scFv mutants."],"pmcid":["PMC8050046"],"funding_grant_id":["19K07021"],"pubmed_authors":["Umezawa N","Oyama H","Kiguchi Y","Morita I","Nagata Y","Kobayashi N"],"additional_accession":[]},"is_claimable":false,"name":"The V<sub>H</sub> framework region 1 as a target of efficient mutagenesis for generating a variety of affinity-matured scFv mutants.","description":"In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targeting \"hot regions,\" i.e., amino acid substitutions therein frequently increase the affinities, is desirable for straightforward discovery of valuable mutants. We here report two \"designed\" site-directed mutagenesis (A and B) targeted the N-terminal 1-10 positions of the V<sub>H</sub> framework region 1 that successfully improved an anti-cortisol single-chain Fv fragment (K<sub>a</sub>, 3.6 × 10<sup>8</sup> M<sup>-1</sup>). Mutagenesis A substituted the amino acids at the position 1-3, 5-7, 9 and 10 with a limited set of substitutions to generate only 1,","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-18T13:20:35.137Z","creation":"2022-02-09T15:56:06.7Z"},"accession":"S-EPMC8050046","cross_references":{"pubmed":["33859250"],"doi":["10.1038/s41598-021-87501-7"]}}