biostudies-literatureSnyder DTNational Institutes of HealthNIH HHSNIGMS NIH HHS116503https://www.ebi.ac.uk/biostudies/studies/S-EPMC8057730biostudies-literatureUnknown461We describe instrumentation for conducting tandem surface-induced dissociation (tSID) of native protein complexes on an ultrahigh resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The two stages of SID are accomplished with split lenses replacing the entrance lenses of the quadrupole mass filter (stage 1, referred to herein as SID-Q) and the collision cell (stage 2, Q-SID). After SID-Q, the scattered projectile ions and subcomplexes formed in transit traverse the 20 mm pre-filter prior to the mass-selecting quadrupole, providing preliminary insights into the SID fragmentation kinetics of noncovalent protein complexes. The isolated SID fragments (subcomplexes) are then fragmented by SID in the collision cell entrance lens (Q-SID), generating subcomplexes of suInternational journal of mass spectrometryTandem surface-induced dissociation of protein complexes on an ultrahigh resolution platform.PMC8057730S10 OD018507P41GM128577R01 GM120425P41 GM128577Wysocki VSnyder DTLin YFSomogyi AfalseTandem surface-induced dissociation of protein complexes on an ultrahigh resolution platform.We describe instrumentation for conducting tandem surface-induced dissociation (tSID) of native protein complexes on an ultrahigh resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The two stages of SID are accomplished with split lenses replacing the entrance lenses of the quadrupole mass filter (stage 1, referred to herein as SID-Q) and the collision cell (stage 2, Q-SID). After SID-Q, the scattered projectile ions and subcomplexes formed in transit traverse the 20 mm pre-filter prior to the mass-selecting quadrupole, providing preliminary insights into the SID fragmentation kinetics of noncovalent protein complexes. The isolated SID fragments (subcomplexes) are then fragmented by SID in the collision cell entrance lens (Q-SID), generating subcomplexes of su2021-01-01T00:00:00Z2021 Mar2026-03-18T13:16:21.048Z2025-04-04T19:58:22.996ZS-EPMC80577303388905510.1016/j.ijms.2020.116503