biostudies-literatureZaveri AMedical Research CouncilDBT/Wellcome Trust India AllianceDepartment of Biotechnology, Ministry of Science and Technology, India1231-1246https://www.ebi.ac.uk/biostudies/studies/S-EPMC8059089biostudies-literatureUnknown120(7)Mycobacteria harbor a unique class of adenylyl cyclases with a complex domain organization consisting of an N-terminal putative adenylyl cyclase domain fused to a nucleotide-binding adaptor shared by apoptotic protease-activating factor-1, plant resistance proteins, and CED-4 (NB-ARC) domain, a tetratricopeptide repeat (TPR) domain, and a C-terminal helix-turn-helix (HTH) domain. The products of the rv0891c-rv0890c genes represent a split gene pair, where Rv0891c has sequence similarity to adenylyl cyclases, and Rv0890c harbors the NB-ARC-TPR-HTH domains. Rv0891c had very low adenylyl cyclase activity so it could represent a pseudoenzyme. By analyzing the genomic locus, we could express and purify Rv0890c and find that the NB-ARC domain binds ATP and ADP, but does not hydrolyze these nucleBiophysical journalMycobacterial STAND adenylyl cyclases: The HTH domain binds DNA to form biocrystallized nucleoids.PMC8059089MR/P028225/1Bose AShenoy ARVisweswariah SSSharma SZaveri ARajendran ABiswas PfalseMycobacterial STAND adenylyl cyclases: The HTH domain binds DNA to form biocrystallized nucleoids.Mycobacteria harbor a unique class of adenylyl cyclases with a complex domain organization consisting of an N-terminal putative adenylyl cyclase domain fused to a nucleotide-binding adaptor shared by apoptotic protease-activating factor-1, plant resistance proteins, and CED-4 (NB-ARC) domain, a tetratricopeptide repeat (TPR) domain, and a C-terminal helix-turn-helix (HTH) domain. The products of the rv0891c-rv0890c genes represent a split gene pair, where Rv0891c has sequence similarity to adenylyl cyclases, and Rv0890c harbors the NB-ARC-TPR-HTH domains. Rv0891c had very low adenylyl cyclase activity so it could represent a pseudoenzyme. By analyzing the genomic locus, we could express and purify Rv0890c and find that the NB-ARC domain binds ATP and ADP, but does not hydrolyze these nucle2021-01-01T00:00:00Z2021 Apr2026-05-31T06:03:26.112Z2025-02-19T01:55:26.874ZS-EPMC80590893321738610.1016/j.bpj.2020.11.008