biostudies-literatureXu NAmerican Heart AssociationNational Heart, Lung, and Blood InstituteNHLBI NIH HHSNational Institutes of HealthU.S. Department of Defense Congressionally Directed Medical Research ProgramNIH HHSe0250095https://www.ebi.ac.uk/biostudies/studies/S-EPMC8064576biostudies-literatureUnknown16(4)Collective migration of endothelial cells is important for wound healing and angiogenesis. During such migration, each constituent endothelial cell coordinates its magnitude and direction of migration with its neighbors while retaining intercellular adhesion. Ensuring coordination and cohesion involves a variety of intra- and inter-cellular signaling processes. However, the role of permeation of extracellular Na+ in collective cell migration remains unclear. Here, we examined the effect of Na+ permeation in collective migration of pulmonary artery endothelial cell (PAEC) monolayers triggered by either a scratch injury or a barrier removal over 24 hours. In the scratch assay, PAEC monolayers migrated in two approximately linear phases. In the first phase, wound closure started with fast spePloS oneImpact of Na+ permeation on collective migration of pulmonary arterial endothelial cells.PMC8064576R37 HL060024HL6002414PRE18470024R01 OD010944W81XWH2110161OD010944S10 OD025089HL066299HL144040P01 HL066299Pastukh VStevens TXu NAlexeyev MAyers LTambe DTfalseImpact of Na+ permeation on collective migration of pulmonary arterial endothelial cells.Collective migration of endothelial cells is important for wound healing and angiogenesis. During such migration, each constituent endothelial cell coordinates its magnitude and direction of migration with its neighbors while retaining intercellular adhesion. Ensuring coordination and cohesion involves a variety of intra- and inter-cellular signaling processes. However, the role of permeation of extracellular Na+ in collective cell migration remains unclear. Here, we examined the effect of Na+ permeation in collective migration of pulmonary artery endothelial cell (PAEC) monolayers triggered by either a scratch injury or a barrier removal over 24 hours. In the scratch assay, PAEC monolayers migrated in two approximately linear phases. In the first phase, wound closure started with fast spe2021-01-01T00:00:00Z20212026-04-07T20:06:57.911Z2022-02-10T09:22:47.269ZS-EPMC80645763389159110.1371/journal.pone.0250095