biostudies-literatureLorenzatti Hiles GMinistry of Science and Technology, TaiwanNational Cancer Institute at the National Institutes of HealthChang Gung Memorial HospitalUniversity of Michigan Undergraduate Research Opportunity ProgramNCI NIH HHSUniversity of Michigan-Chang Gung Memorial Hospital Pilot Grante0250530https://www.ebi.ac.uk/biostudies/studies/S-EPMC8064583biostudies-literatureUnknown16(4)<h4>Background and objectives</h4>Human papillomavirus (HPV)-driven oropharyngeal squamous cell carcinoma (OPSCC) is increasing globally. In Taiwan, HPV-positive OPSCC is obscured by tobacco, alcohol, and betel quid use. We investigated the role of high-risk HPV (hrHPV) in a large retrospective Taiwan OPSCC cohort.<h4>Methods and results</h4>The cohort of 541 OPSCCs treated at Chang Gung Memorial Hospital from 1998-2016 consisted of 507 men (94%) and 34 women (6%). Most used tobacco (81%), alcohol (51%), and betel quid (65%). Formalin-fixed, paraffin-embedded tissue was used for p16 staining (a surrogate marker for HPV) and testing for HPV DNA presence and type by Multiplex HPV PCR-MassArray. HPV DNA and/or p16 staining (HPV-positive) was found in 28.4% (150/528) tumors. p16 and HPV DNA wePloS oneUnderstanding the impact of high-risk human papillomavirus on oropharyngeal squamous cell carcinomas in Taiwan: A retrospective cohort study.PMC8064583CORPG3G0171CMRPG3H0852MOST 108-2314-B-182A-108-MY3CMRPG3J1251R01 CA194536CA194536CA194536-S1Wang CIPinatti LMWalline HMThomas TBGoudsmit CMChang KPYen WCAfsari MACarey TEBellile ELMorris ACBriggs HLPeters LJawad NLorenzatti Hiles GfalseUnderstanding the impact of high-risk human papillomavirus on oropharyngeal squamous cell carcinomas in Taiwan: A retrospective cohort study.<h4>Background and objectives</h4>Human papillomavirus (HPV)-driven oropharyngeal squamous cell carcinoma (OPSCC) is increasing globally. In Taiwan, HPV-positive OPSCC is obscured by tobacco, alcohol, and betel quid use. We investigated the role of high-risk HPV (hrHPV) in a large retrospective Taiwan OPSCC cohort.<h4>Methods and results</h4>The cohort of 541 OPSCCs treated at Chang Gung Memorial Hospital from 1998-2016 consisted of 507 men (94%) and 34 women (6%). Most used tobacco (81%), alcohol (51%), and betel quid (65%). Formalin-fixed, paraffin-embedded tissue was used for p16 staining (a surrogate marker for HPV) and testing for HPV DNA presence and type by Multiplex HPV PCR-MassArray. HPV DNA and/or p16 staining (HPV-positive) was found in 28.4% (150/528) tumors. p16 and HPV DNA we2021-01-01T00:00:00Z20212026-06-03T19:27:08.434Z2025-02-19T03:49:18.342ZS-EPMC80645833389162710.1371/journal.pone.0250530