{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Yu W"],"pubmed_abstract":["<h4>Objective</h4><i>XPG</i> (Xeroderma pigmentosum group G, <i>XPG</i>), a single strand-specific DNA endonuclease in the nucleotide excision repair pathway, has been implicated in lung cancer. Potentially functional rs873601 in <i>XPG</i> is consistently associated with gastrointestinal cancer, and miR-4715-3p, targeting 3UTR of <i>XPG</i>, also influences the process of gastrointestinal carcinogenesis, however, the relationships between <i>XPG</i> and miR-4715-3p and rs873601 in lung cancer have not been elucidated.<h4>Methods</h4>A case-control study included 264 lung cancer patients and 264 cancer-free healthy controls and was designed to determine the relationships between rs873601 and lung cancer and the effect of miR-4715-3p on <i>XPG</i> expression in lung cancer. Fifty matched cases and controls were randomly selected from the lung cancer and control groups to assess the relationships between the expression levels of miR-4715-3p and <i>XPG</i> determined by using qRT-PCR. The association of rs873601 with lung cancer was analyzed by mass spectrometry, and function prediciton of rs873601 genotypes explored by web-based bioinformatics.<h4>Results</h4>miR-4715-3p in the lung cancer group was significantly increased compared with that in the control group (<i>P</i> = 0.011), upregulation of miR-4715-3p correlated with an increase in <i>XPG</i> mRNA (r = 0.399, <i>P</i> <0.05) in the lung cancer group. The AA genotype was associated with increased risk of lung cancer compared with the AG and GG genotypes of rs873601 (AG vs AA: OR = 0.231, 95% CI: 0.155-0.345, <i>P</i> <0.001 GG vs AA: OR = 0.300, 95% CI: 0.131-0.719, <i>P</i> = 0.003). The genetic association remained significant after adjustment for age, sex, smoking, and drinking, and rs873601-AA was associated with an increase in <i>XPG</i> mRNA in the lung cancer group. The results of web-based bioinformatics analysis indicated rs873601 genotypes might change XPG-RNA stability and bindability between XPG and miR-4715-3p.<h4>Conclusion</h4>Our data characterized that miR-4715-3p and rs873601 genotypes modified <i>XPG</i> expression in lung cancer. These findings may help to elucidate the mechanisms governing lung cancer."],"journal":["Cancer management and research"],"pagination":["3417-3427"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8064622"],"repository":["biostudies-literature"],"pubmed_title":["XPG is Modulated by miR-4715-3p and rs873601 Genotypes in Lung Cancer."],"pmcid":["PMC8064622"],"pubmed_authors":["Liu X","Yao J","Yu W","Chen X","Xiao S","Lyu P","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"XPG is Modulated by miR-4715-3p and rs873601 Genotypes in Lung Cancer.","description":"<h4>Objective</h4><i>XPG</i> (Xeroderma pigmentosum group G, <i>XPG</i>), a single strand-specific DNA endonuclease in the nucleotide excision repair pathway, has been implicated in lung cancer. Potentially functional rs873601 in <i>XPG</i> is consistently associated with gastrointestinal cancer, and miR-4715-3p, targeting 3UTR of <i>XPG</i>, also influences the process of gastrointestinal carcinogenesis, however, the relationships between <i>XPG</i> and miR-4715-3p and rs873601 in lung cancer have not been elucidated.<h4>Methods</h4>A case-control study included 264 lung cancer patients and 264 cancer-free healthy controls and was designed to determine the relationships between rs873601 and lung cancer and the effect of miR-4715-3p on <i>XPG</i> expression in lung cancer. Fifty matched cases and controls were randomly selected from the lung cancer and control groups to assess the relationships between the expression levels of miR-4715-3p and <i>XPG</i> determined by using qRT-PCR. The association of rs873601 with lung cancer was analyzed by mass spectrometry, and function prediciton of rs873601 genotypes explored by web-based bioinformatics.<h4>Results</h4>miR-4715-3p in the lung cancer group was significantly increased compared with that in the control group (<i>P</i> = 0.011), upregulation of miR-4715-3p correlated with an increase in <i>XPG</i> mRNA (r = 0.399, <i>P</i> <0.05) in the lung cancer group. The AA genotype was associated with increased risk of lung cancer compared with the AG and GG genotypes of rs873601 (AG vs AA: OR = 0.231, 95% CI: 0.155-0.345, <i>P</i> <0.001 GG vs AA: OR = 0.300, 95% CI: 0.131-0.719, <i>P</i> = 0.003). The genetic association remained significant after adjustment for age, sex, smoking, and drinking, and rs873601-AA was associated with an increase in <i>XPG</i> mRNA in the lung cancer group. The results of web-based bioinformatics analysis indicated rs873601 genotypes might change XPG-RNA stability and bindability between XPG and miR-4715-3p.<h4>Conclusion</h4>Our data characterized that miR-4715-3p and rs873601 genotypes modified <i>XPG</i> expression in lung cancer. These findings may help to elucidate the mechanisms governing lung cancer.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2022-02-09T18:03:39.589Z","creation":"2022-02-09T18:03:39.589Z"},"accession":"S-EPMC8064622","cross_references":{"pubmed":["33907465"],"doi":["10.2147/CMAR.S294365"]}}