{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Puccio T"],"funding":["National Institute of Allergy and Infectious Diseases","NIDCR NIH HHS","NIAID NIH HHS","National Institute of Dental and Craniofacial Research"],"pagination":["44"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8064989"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(5)"],"pubmed_abstract":["<h4>Introduction</h4>Manganese is important for the endocarditis pathogen Streptococcus sanguinis. Little is known about why manganese is required for virulence or how it impacts the metabolome of streptococci.<h4>Objectives</h4>We applied untargeted metabolomics to cells and media to understand temporal changes resulting from manganese depletion.<h4>Methods</h4>EDTA was added to a S. sanguinis manganese-transporter mutant in aerobic fermentor conditions. Cell and media samples were collected pre- and post-EDTA treatment. Metabolomics data were generated using positive and negative modes of data acquisition on an LC-MS/MS system. Data were subjected to statistical processing using MetaboAnalyst and time-course analysis using Short Time series Expression Miner (STEM). Recombinant enzymes we"],"journal":["Metabolomics : Official journal of the Metabolomic Society"],"pubmed_title":["Time-course analysis of Streptococcus sanguinis after manganese depletion reveals changes in glycolytic and nucleic acid metabolites."],"pmcid":["PMC8064989"],"funding_grant_id":["F31DE028468","F31 DE028468","R01 AI114926","R01AI114926"],"pubmed_authors":["Kitten T","Misra BB","Puccio T"],"additional_accession":[]},"is_claimable":false,"name":"Time-course analysis of Streptococcus sanguinis after manganese depletion reveals changes in glycolytic and nucleic acid metabolites.","description":"<h4>Introduction</h4>Manganese is important for the endocarditis pathogen Streptococcus sanguinis. Little is known about why manganese is required for virulence or how it impacts the metabolome of streptococci.<h4>Objectives</h4>We applied untargeted metabolomics to cells and media to understand temporal changes resulting from manganese depletion.<h4>Methods</h4>EDTA was added to a S. sanguinis manganese-transporter mutant in aerobic fermentor conditions. Cell and media samples were collected pre- and post-EDTA treatment. Metabolomics data were generated using positive and negative modes of data acquisition on an LC-MS/MS system. Data were subjected to statistical processing using MetaboAnalyst and time-course analysis using Short Time series Expression Miner (STEM). Recombinant enzymes we","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-18T16:51:27.854Z","creation":"2025-04-07T04:18:20.119Z"},"accession":"S-EPMC8064989","cross_references":{"pubmed":["33893555"],"doi":["10.1007/s11306-021-01795-2"]}}