biostudies-literaturePuccio TNational Institute of Allergy and Infectious DiseasesNIDCR NIH HHSNIAID NIH HHSNational Institute of Dental and Craniofacial Research44https://www.ebi.ac.uk/biostudies/studies/S-EPMC8064989biostudies-literatureUnknown17(5)<h4>Introduction</h4>Manganese is important for the endocarditis pathogen Streptococcus sanguinis. Little is known about why manganese is required for virulence or how it impacts the metabolome of streptococci.<h4>Objectives</h4>We applied untargeted metabolomics to cells and media to understand temporal changes resulting from manganese depletion.<h4>Methods</h4>EDTA was added to a S. sanguinis manganese-transporter mutant in aerobic fermentor conditions. Cell and media samples were collected pre- and post-EDTA treatment. Metabolomics data were generated using positive and negative modes of data acquisition on an LC-MS/MS system. Data were subjected to statistical processing using MetaboAnalyst and time-course analysis using Short Time series Expression Miner (STEM). Recombinant enzymes weMetabolomics : Official journal of the Metabolomic SocietyTime-course analysis of Streptococcus sanguinis after manganese depletion reveals changes in glycolytic and nucleic acid metabolites.PMC8064989F31DE028468F31 DE028468R01 AI114926R01AI114926Kitten TMisra BBPuccio TfalseTime-course analysis of Streptococcus sanguinis after manganese depletion reveals changes in glycolytic and nucleic acid metabolites.<h4>Introduction</h4>Manganese is important for the endocarditis pathogen Streptococcus sanguinis. Little is known about why manganese is required for virulence or how it impacts the metabolome of streptococci.<h4>Objectives</h4>We applied untargeted metabolomics to cells and media to understand temporal changes resulting from manganese depletion.<h4>Methods</h4>EDTA was added to a S. sanguinis manganese-transporter mutant in aerobic fermentor conditions. Cell and media samples were collected pre- and post-EDTA treatment. Metabolomics data were generated using positive and negative modes of data acquisition on an LC-MS/MS system. Data were subjected to statistical processing using MetaboAnalyst and time-course analysis using Short Time series Expression Miner (STEM). Recombinant enzymes we2021-01-01T00:00:00Z2021 Apr2025-04-18T16:51:27.854Z2025-04-07T04:18:20.119ZS-EPMC80649893389355510.1007/s11306-021-01795-2