{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song Y"],"funding":["NINDS NIH HHS"],"pagination":["1591-1602"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8065241"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["137(12)"],"pubmed_abstract":["Digital protein assays have great potential to advance immunodiagnostics because of their single-molecule sensitivity, high precision, and robust measurements. However, translating digital protein assays to acute clinical care has been challenging because it requires deployment of these assays with a rapid turnaround. Herein, we present a technology platform for ultrafast digital protein biomarker detection by using single-molecule counting of immune-complex formation events at an early, pre-equilibrium state. This method, which we term \"pre-equilibrium digital enzyme-linked immunosorbent assay\" (PEdELISA), can quantify a multiplexed panel of protein biomarkers in 10 µL of serum within an unprecedented assay incubation time of 15 to 300 seconds over a 104 dynamic range. PEdELISA allowed us to perform rapid monitoring of protein biomarkers in patients manifesting post-chimeric antigen receptor T-cell therapy cytokine release syndrome, with ∼30-minute sample-to-answer time and a sub-picograms per mL limit of detection. The rapid, sensitive, and low-input volume biomarker quantification enabled by PEdELISA is broadly applicable to timely monitoring of acute disease, potentially enabling more personalized treatment."],"journal":["Blood"],"pubmed_title":["Rapid single-molecule digital detection of protein biomarkers for continuous monitoring of systemic immune disorders."],"pmcid":["PMC8065241"],"funding_grant_id":["K08 NS101054"],"pubmed_authors":["Choi SW","Cai T","Kozminski AG","Chung MT","Barabas J","Sandford E","Alam HB","Tewari M","Ye Y","Li Y","Su SH","Ghosh M","Lindstrom R","Singer BH","Tian Y","Olesnavich M","Rozwadowski M","Yin Q","Goicochea A","Song Y","Kurabayashi K"],"additional_accession":[]},"is_claimable":false,"name":"Rapid single-molecule digital detection of protein biomarkers for continuous monitoring of systemic immune disorders.","description":"Digital protein assays have great potential to advance immunodiagnostics because of their single-molecule sensitivity, high precision, and robust measurements. However, translating digital protein assays to acute clinical care has been challenging because it requires deployment of these assays with a rapid turnaround. Herein, we present a technology platform for ultrafast digital protein biomarker detection by using single-molecule counting of immune-complex formation events at an early, pre-equilibrium state. This method, which we term \"pre-equilibrium digital enzyme-linked immunosorbent assay\" (PEdELISA), can quantify a multiplexed panel of protein biomarkers in 10 µL of serum within an unprecedented assay incubation time of 15 to 300 seconds over a 104 dynamic range. PEdELISA allowed us to perform rapid monitoring of protein biomarkers in patients manifesting post-chimeric antigen receptor T-cell therapy cytokine release syndrome, with ∼30-minute sample-to-answer time and a sub-picograms per mL limit of detection. The rapid, sensitive, and low-input volume biomarker quantification enabled by PEdELISA is broadly applicable to timely monitoring of acute disease, potentially enabling more personalized treatment.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2024-11-09T22:22:21.578Z","creation":"2022-02-09T18:03:57.126Z"},"accession":"S-EPMC8065241","cross_references":{"pubmed":["33275650"],"doi":["10.1182/blood.2019004399"]}}