{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Heidepriem J"],"funding":["Bundesministerium für Bildung und Forschung","German Center for Infection Research","MPG-FhG cooperation"],"pagination":["438"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8067489"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(4)"],"pubmed_abstract":["The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). A better understanding of its immunogenicity can be important for the development of improved diagnostics, therapeutics, and vaccines. Here, we report the longitudinal analysis of three COVID-19 patients with moderate (#1) and mild disease (#2 and #3). Antibody serum responses were analyzed using spike glycoprotein enzyme linked immunosorbent assay (ELISA), full-proteome peptide, and glycan microarrays. ELISA immunoglobulin A, G, and M (IgA, IgG, and IgM) signals increased over time for individuals #1 and #2, whereas #3 only showed no clear positive IgG and IgM result. In contrast, peptide microarrays showed increasing IgA/G signal intensity and epitope spread only in the moderate p"],"journal":["Pathogens (Basel, Switzerland)"],"pubmed_title":["Longitudinal Development of Antibody Responses in COVID-19 Patients of Different Severity with ELISA, Peptide, and Glycan Arrays: An Immunological Case Series."],"pmcid":["PMC8067489"],"funding_grant_id":["Glyco3Display","DZIF TTU01921","13XP5050A"],"pubmed_authors":["On Behalf Of The Id-Uke Covid-Study Group","Kobbe R","Seeberger PH","Sellrie K","Heidepriem J","Seco BMS","Ly ML","Reichardt NC","Dahlke C","Santer R","Fathi A","Loeffler FF","Schwinge D","Koch T","Serna S","Schmiedel S","Addo MM"],"additional_accession":[]},"is_claimable":false,"name":"Longitudinal Development of Antibody Responses in COVID-19 Patients of Different Severity with ELISA, Peptide, and Glycan Arrays: An Immunological Case Series.","description":"The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). A better understanding of its immunogenicity can be important for the development of improved diagnostics, therapeutics, and vaccines. Here, we report the longitudinal analysis of three COVID-19 patients with moderate (#1) and mild disease (#2 and #3). Antibody serum responses were analyzed using spike glycoprotein enzyme linked immunosorbent assay (ELISA), full-proteome peptide, and glycan microarrays. ELISA immunoglobulin A, G, and M (IgA, IgG, and IgM) signals increased over time for individuals #1 and #2, whereas #3 only showed no clear positive IgG and IgM result. In contrast, peptide microarrays showed increasing IgA/G signal intensity and epitope spread only in the moderate p","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-04-07T23:33:19.796Z","creation":"2022-02-10T10:23:50.767Z"},"accession":"S-EPMC8067489","cross_references":{"pubmed":["33917609"],"doi":["10.3390/pathogens10040438"]}}