biostudies-literatureHeidepriem JBundesministerium für Bildung und ForschungGerman Center for Infection ResearchMPG-FhG cooperation438https://www.ebi.ac.uk/biostudies/studies/S-EPMC8067489biostudies-literatureUnknown10(4)The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). A better understanding of its immunogenicity can be important for the development of improved diagnostics, therapeutics, and vaccines. Here, we report the longitudinal analysis of three COVID-19 patients with moderate (#1) and mild disease (#2 and #3). Antibody serum responses were analyzed using spike glycoprotein enzyme linked immunosorbent assay (ELISA), full-proteome peptide, and glycan microarrays. ELISA immunoglobulin A, G, and M (IgA, IgG, and IgM) signals increased over time for individuals #1 and #2, whereas #3 only showed no clear positive IgG and IgM result. In contrast, peptide microarrays showed increasing IgA/G signal intensity and epitope spread only in the moderate pPathogens (Basel, Switzerland)Longitudinal Development of Antibody Responses in COVID-19 Patients of Different Severity with ELISA, Peptide, and Glycan Arrays: An Immunological Case Series.PMC8067489Glyco3DisplayDZIF TTU0192113XP5050AOn Behalf Of The Id-Uke Covid-Study GroupKobbe RSeeberger PHSellrie KHeidepriem JSeco BMSLy MLReichardt NCDahlke CSanter RFathi ALoeffler FFSchwinge DKoch TSerna SSchmiedel SAddo MMfalseLongitudinal Development of Antibody Responses in COVID-19 Patients of Different Severity with ELISA, Peptide, and Glycan Arrays: An Immunological Case Series.The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). A better understanding of its immunogenicity can be important for the development of improved diagnostics, therapeutics, and vaccines. Here, we report the longitudinal analysis of three COVID-19 patients with moderate (#1) and mild disease (#2 and #3). Antibody serum responses were analyzed using spike glycoprotein enzyme linked immunosorbent assay (ELISA), full-proteome peptide, and glycan microarrays. ELISA immunoglobulin A, G, and M (IgA, IgG, and IgM) signals increased over time for individuals #1 and #2, whereas #3 only showed no clear positive IgG and IgM result. In contrast, peptide microarrays showed increasing IgA/G signal intensity and epitope spread only in the moderate p2021-01-01T00:00:00Z2021 Apr2026-04-07T23:33:19.796Z2022-02-10T10:23:50.767ZS-EPMC80674893391760910.3390/pathogens10040438