{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["22(8)"],"submitter":["Wang J"],"pubmed_abstract":["Tubules of the endoplasmic reticulum (ER) spread into the buds of yeast by an actin-based mechanism and, upon entry, become attached to the polarisome, a proteinaceous micro-compartment below the tip of the bud. The minimal tether between polarisome and cortical ER is formed by a protein complex consisting of Epo1, a member of the polarisome, Scs2, a membrane protein of the ER and Cdc42 guanosine triphosphatase-activating protein Bem3. Here, we report the crystal structure of a complex between Epo1 and Bem3. In addition, we characterize through the hydrogen/deuterium (H/D) exchange assay the interface between Scs2 and Epo1. Our findings provide a first structural insight into the molecular architecture of the link between cortical ER and the polarisome."],"journal":["International journal of molecular sciences"],"pagination":["3812"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8067709"],"repository":["biostudies-literature"],"pubmed_title":["Crystal Structure of the Epo1-Bem3 Complex for Bud Growth."],"pmcid":["PMC8067709"],"pubmed_authors":["Yan L","Wu L","Wang J","Li L","Ming Z"],"additional_accession":[]},"is_claimable":false,"name":"Crystal Structure of the Epo1-Bem3 Complex for Bud Growth.","description":"Tubules of the endoplasmic reticulum (ER) spread into the buds of yeast by an actin-based mechanism and, upon entry, become attached to the polarisome, a proteinaceous micro-compartment below the tip of the bud. The minimal tether between polarisome and cortical ER is formed by a protein complex consisting of Epo1, a member of the polarisome, Scs2, a membrane protein of the ER and Cdc42 guanosine triphosphatase-activating protein Bem3. Here, we report the crystal structure of a complex between Epo1 and Bem3. In addition, we characterize through the hydrogen/deuterium (H/D) exchange assay the interface between Scs2 and Epo1. Our findings provide a first structural insight into the molecular architecture of the link between cortical ER and the polarisome.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-05-08T15:00:15.085Z","creation":"2026-04-08T00:03:13.817Z"},"accession":"S-EPMC8067709","cross_references":{"pubmed":["33917059"],"doi":["10.3390/ijms22083812"]}}