{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(4)"],"submitter":["Kayushin AL"],"pubmed_abstract":["During the preparative synthesis of 2-fluorocordycepin from 2-fluoroadenosine and 3'-deoxyinosine catalyzed by <i>E. coli</i> purine nucleoside phosphorylase, a slowdown of the reaction and decrease of yield down to 5% were encountered. An unknown nucleoside was found in the reaction mixture and its structure was established. This nucleoside is formed from the admixture of 2',3'-anhydroinosine, a byproduct in the preparation of 3-'deoxyinosine. Moreover, 2',3'-anhydroinosine forms during radical dehalogenation of 9-(2',5'-di-<i>O</i>-acetyl-3'-bromo- -3'-deoxyxylofuranosyl)hypoxanthine, a precursor of 3'-deoxyinosine in chemical synthesis. The products of 2',3'-anhydroinosine hydrolysis inhibit the formation of 1-phospho-3-deoxyribose during the synthesis of 2-fluorocordycepin. The progres"],"journal":["Biomolecules"],"pagination":["539"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8067715"],"repository":["biostudies-literature"],"pubmed_title":["Radical Dehalogenation and Purine Nucleoside Phosphorylase <i>E. coli</i>: How Does an Admixture of 2',3'-Anhydroinosine Hinder 2-fluoro-cordycepin Synthesis."],"pmcid":["PMC8067715"],"pubmed_authors":["Dorofeeva EV","Miroshnikov AI","Lutonina OI","Fateev IV","Kayushin AL","Antonov KV","Tokunova JA","Berzina MY","Paramonov AS","Arnautova AO","Esipov RS","Mikhailopulo IA","Konstantinova ID"],"additional_accession":[]},"is_claimable":false,"name":"Radical Dehalogenation and Purine Nucleoside Phosphorylase <i>E. coli</i>: How Does an Admixture of 2',3'-Anhydroinosine Hinder 2-fluoro-cordycepin Synthesis.","description":"During the preparative synthesis of 2-fluorocordycepin from 2-fluoroadenosine and 3'-deoxyinosine catalyzed by <i>E. coli</i> purine nucleoside phosphorylase, a slowdown of the reaction and decrease of yield down to 5% were encountered. An unknown nucleoside was found in the reaction mixture and its structure was established. This nucleoside is formed from the admixture of 2',3'-anhydroinosine, a byproduct in the preparation of 3-'deoxyinosine. Moreover, 2',3'-anhydroinosine forms during radical dehalogenation of 9-(2',5'-di-<i>O</i>-acetyl-3'-bromo- -3'-deoxyxylofuranosyl)hypoxanthine, a precursor of 3'-deoxyinosine in chemical synthesis. The products of 2',3'-anhydroinosine hydrolysis inhibit the formation of 1-phospho-3-deoxyribose during the synthesis of 2-fluorocordycepin. The progres","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-07-16T06:22:43.326Z","creation":"2025-05-31T23:33:04.647Z"},"accession":"S-EPMC8067715","cross_references":{"pubmed":["33917025"],"doi":["10.3390/biom11040539"]}}