biostudies-literatureUnknown12Liu YWu Jieping Medical FoundationNatural Science Foundation of ShanghaiInterleukin (IL)-35-secreting B (IL-35+B) cells are critical regulators in autoimmune and infectious diseases and exert suppressive functions in parallel with IL-10-producing B (B10) cells. However, the role of IL-35+B cells in persistent hepatitis B virus (HBV) infection remains unclear. To elucidate the role of IL-35+B cells in the progress of chronic HBV infection, we determined the frequency of IL-35+B cells and their relationship with the classical human regulatory B cell (Breg) subsets, namely, CD19+CD24^hiCD38^hi and CD19+CD24^hiCD27+. Then, the regulatory effect and mechanism of Bregs on effector T cells were investigated <i>in vitro</i>. Here, we found that compared with healthy controls, the frequency of IL-35+B cells was increased in patients with cFrontiers in immunology653198https://www.ebi.ac.uk/biostudies/studies/S-EPMC8072152biostudies-literatureRegulatory B Cells Dysregulated T Cell Function in an IL-35-Dependent Way in Patients With Chronic Hepatitis B.PMC8072152Tian ZLiu YLiang XLuo YJiang MTang GZhu TfalseRegulatory B Cells Dysregulated T Cell Function in an IL-35-Dependent Way in Patients With Chronic Hepatitis B.Interleukin (IL)-35-secreting B (IL-35+B) cells are critical regulators in autoimmune and infectious diseases and exert suppressive functions in parallel with IL-10-producing B (B10) cells. However, the role of IL-35+B cells in persistent hepatitis B virus (HBV) infection remains unclear. To elucidate the role of IL-35+B cells in the progress of chronic HBV infection, we determined the frequency of IL-35+B cells and their relationship with the classical human regulatory B cell (Breg) subsets, namely, CD19+CD24^hiCD38^hi and CD19+CD24^hiCD27+. Then, the regulatory effect and mechanism of Bregs on effector T cells were investigated <i>in vitro</i>. Here, we found that compared with healthy controls, the frequency of IL-35+B cells was increased in patients with c2021-01-01T00:00:00Z20212026-05-03T09:06:10.866Z2022-02-10T08:21:47.739ZS-EPMC80721523391217810.3389/fimmu.2021.653198