{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(1)"],"submitter":["Nived P"],"funding":["Medicinska Fakulteten, Lunds Universitet","Wiberg Foundation","Anna-Greta Crafoord Foundation","Professor Nanna Svartz Foundation","Maggie Stephens Foundation","Lund University","Greta och Johan Kocks stiftelser","Swedish Rheumatism Association","Alfred Österlunds Stiftelse","Skånes universitetssjukhus, Lund, Sweden","The King Gustaf V Foundation","The Research and Development Committee at the Central Hospital Kristianstad","Inger Bendix Foundation","Apotekare Hedbergs Foundation"],"pubmed_abstract":["Methotrexate (MTX) impairs antibody response after pneumococcal vaccination. We aimed to investigate differences in phenotypes of circulating B and T cells after pneumococcal conjugate vaccine (PCV) in rheumatoid arthritis (RA) patients on MTX (MTX group), RA without disease-modifying drugs (0DMARD), and controls (HC). MTX group (n = 11), 0DMARD (n = 12) and HC (n = 13) were studied. Blood samples were collected: before MTX, ≥ 4 weeks on stable MTX dose (prevaccination), and 7 days postvaccination (MTX group), and pre- and 7 days postvaccination (0DMARD and HC). Phenotypes of B- and T cell subsets were determined using flow cytometry. Serotype-specific IgG were quantified using multiplex bead assay, pre- and 4-6 weeks postvaccination. Concentrations of plasmablasts and switched memory B ce"],"journal":["Scientific reports"],"pagination":["9199"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8080705"],"repository":["biostudies-literature"],"pubmed_title":["Methotrexate reduces circulating Th17 cells and impairs plasmablast and memory B cell expansions following pneumococcal conjugate immunization in RA patients."],"pmcid":["PMC8080705"],"pubmed_authors":["Jonsson G","Kapetanovic MC","Bengtsson AA","Johansson A","Settergren B","Pettersson A","Skattum L","Nived P"],"additional_accession":[]},"is_claimable":false,"name":"Methotrexate reduces circulating Th17 cells and impairs plasmablast and memory B cell expansions following pneumococcal conjugate immunization in RA patients.","description":"Methotrexate (MTX) impairs antibody response after pneumococcal vaccination. We aimed to investigate differences in phenotypes of circulating B and T cells after pneumococcal conjugate vaccine (PCV) in rheumatoid arthritis (RA) patients on MTX (MTX group), RA without disease-modifying drugs (0DMARD), and controls (HC). MTX group (n = 11), 0DMARD (n = 12) and HC (n = 13) were studied. Blood samples were collected: before MTX, ≥ 4 weeks on stable MTX dose (prevaccination), and 7 days postvaccination (MTX group), and pre- and 7 days postvaccination (0DMARD and HC). Phenotypes of B- and T cell subsets were determined using flow cytometry. Serotype-specific IgG were quantified using multiplex bead assay, pre- and 4-6 weeks postvaccination. Concentrations of plasmablasts and switched memory B ce","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2026-05-09T01:06:06.431Z","creation":"2025-05-29T22:19:16.66Z"},"accession":"S-EPMC8080705","cross_references":{"pubmed":["33911135"],"doi":["10.1038/s41598-021-88491-2"]}}