biostudies-literatureUnknown11(1)Nived PMedicinska Fakulteten, Lunds UniversitetWiberg FoundationAnna-Greta Crafoord FoundationProfessor Nanna Svartz FoundationMaggie Stephens FoundationLund UniversityGreta och Johan Kocks stiftelserSwedish Rheumatism AssociationAlfred Österlunds StiftelseSkånes universitetssjukhus, Lund, SwedenThe King Gustaf V FoundationThe Research and Development Committee at the Central Hospital KristianstadInger Bendix FoundationApotekare Hedbergs FoundationMethotrexate (MTX) impairs antibody response after pneumococcal vaccination. We aimed to investigate differences in phenotypes of circulating B and T cells after pneumococcal conjugate vaccine (PCV) in rheumatoid arthritis (RA) patients on MTX (MTX group), RA without disease-modifying drugs (0DMARD), and controls (HC). MTX group (n = 11), 0DMARD (n = 12) and HC (n = 13) were studied. Blood samples were collected: before MTX, ≥ 4 weeks on stable MTX dose (prevaccination), and 7 days postvaccination (MTX group), and pre- and 7 days postvaccination (0DMARD and HC). Phenotypes of B- and T cell subsets were determined using flow cytometry. Serotype-specific IgG were quantified using multiplex bead assay, pre- and 4-6 weeks postvaccination. Concentrations of plasmablasts and switched memory B ceScientific reports9199https://www.ebi.ac.uk/biostudies/studies/S-EPMC8080705biostudies-literatureMethotrexate reduces circulating Th17 cells and impairs plasmablast and memory B cell expansions following pneumococcal conjugate immunization in RA patients.PMC8080705Jonsson GKapetanovic MCBengtsson AAJohansson ASettergren BPettersson ASkattum LNived PfalseMethotrexate reduces circulating Th17 cells and impairs plasmablast and memory B cell expansions following pneumococcal conjugate immunization in RA patients.Methotrexate (MTX) impairs antibody response after pneumococcal vaccination. We aimed to investigate differences in phenotypes of circulating B and T cells after pneumococcal conjugate vaccine (PCV) in rheumatoid arthritis (RA) patients on MTX (MTX group), RA without disease-modifying drugs (0DMARD), and controls (HC). MTX group (n = 11), 0DMARD (n = 12) and HC (n = 13) were studied. Blood samples were collected: before MTX, ≥ 4 weeks on stable MTX dose (prevaccination), and 7 days postvaccination (MTX group), and pre- and 7 days postvaccination (0DMARD and HC). Phenotypes of B- and T cell subsets were determined using flow cytometry. Serotype-specific IgG were quantified using multiplex bead assay, pre- and 4-6 weeks postvaccination. Concentrations of plasmablasts and switched memory B ce2021-01-01T00:00:00Z2021 Apr2026-05-09T01:06:06.431Z2025-05-29T22:19:16.66ZS-EPMC80807053391113510.1038/s41598-021-88491-2