biostudies-literatureValle BLNIMHD NIH HHSNCI NIH HHS783-794https://www.ebi.ac.uk/biostudies/studies/S-EPMC8082233biostudies-literatureUnknown13(9)Molecular alterations that contribute to long-term (LT) and short-term (ST) survival in ovarian high-grade serous carcinoma (HGSC) may be used as precision medicine biomarkers. DNA promoter methylation is an early event in tumorigenesis, which can be detected in blood and urine, making it a feasible companion biomarker to somatic mutations for early detection and targeted treatment workflows. We compared the methylation profile in 12 HGSC tissue samples to 30 fallopian tube epithelium samples, using the Infinium Human Methylation 450K Array. We also used 450K methylation arrays to compare methylation among HGSCs long-term survivors (more than 5 years) and short-term survivors (less than 3 years). We verified the array results using bisulfite sequencing and methylation-specific PCR (qMSP). Cancer prevention research (Philadelphia, Pa.)<i>HIST1H2BB</i> and <i>MAGI2</i> Methylation and Somatic Mutations as Precision Medicine Biomarkers for Diagnosis and Prognosis of High-grade Serous Ovarian Cancer.PMC8082233K01 CA164092U01 CA084986R44 MD014911P50 CA228991Rodriguez-Torres SShih IMIli-Gangas CKuhn ESidransky DEshleman JRLawson FPHerman JParrilla-Castellar EBrebi-Mieville PDiaz-Montes TFolawiyo OGuerrero-Preston RValle BLfalse<i>HIST1H2BB</i> and <i>MAGI2</i> Methylation and Somatic Mutations as Precision Medicine Biomarkers for Diagnosis and Prognosis of High-grade Serous Ovarian Cancer.Molecular alterations that contribute to long-term (LT) and short-term (ST) survival in ovarian high-grade serous carcinoma (HGSC) may be used as precision medicine biomarkers. DNA promoter methylation is an early event in tumorigenesis, which can be detected in blood and urine, making it a feasible companion biomarker to somatic mutations for early detection and targeted treatment workflows. We compared the methylation profile in 12 HGSC tissue samples to 30 fallopian tube epithelium samples, using the Infinium Human Methylation 450K Array. We also used 450K methylation arrays to compare methylation among HGSCs long-term survivors (more than 5 years) and short-term survivors (less than 3 years). We verified the array results using bisulfite sequencing and methylation-specific PCR (qMSP). 2020-01-01T00:00:00Z2020 Sep2026-05-08T13:30:21.071Z2022-02-10T08:19:37.836ZS-EPMC80822333258101010.1158/1940-6207.capr-19-041210.1158/1940-6207.CAPR-19-0412