biostudies-literatureRank LNIAID NIH HHSHHS | NIH | National Cancer InstituteNCI NIH HHSHHS | NIH | National Institute of Allergy and Infectious Diseasese00685-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC8088607biostudies-literatureUnknown203(10)Mycobacteria possess Mce transporters that import lipids and are thought to function analogously to ATP-binding cassette (ABC) transporters. However, whereas ABC transporters import substrates using a single solute-binding protein (SBP) to deliver a substrate to permease proteins in the membrane, mycobacterial Mce transporters have a potential for six SBPs (MceA to MceF) working with a pair of permeases (YrbEA and YrbEB), a cytoplasmic ATPase (MceG), and multiple Mce-associated membrane (Mam) and orphaned Mam (Omam) proteins to transport lipids. In this study, we used the model mycobacterium <i>Mycobacterium smegmatis</i> to study the requirement for individual Mce, Mam, and Omam proteins in Mce4 transport of cholesterol. All of the Mce4 and Mam4 proteins we investigated were required for Journal of bacteriologyEvidence for the Mycobacterial Mce4 Transporter Being a Multiprotein Complex.PMC8088607P30CA016086P30 CA016086AI135899R21 AI135899Braunstein MHerring LERank LfalseEvidence for the Mycobacterial Mce4 Transporter Being a Multiprotein Complex.Mycobacteria possess Mce transporters that import lipids and are thought to function analogously to ATP-binding cassette (ABC) transporters. However, whereas ABC transporters import substrates using a single solute-binding protein (SBP) to deliver a substrate to permease proteins in the membrane, mycobacterial Mce transporters have a potential for six SBPs (MceA to MceF) working with a pair of permeases (YrbEA and YrbEB), a cytoplasmic ATPase (MceG), and multiple Mce-associated membrane (Mam) and orphaned Mam (Omam) proteins to transport lipids. In this study, we used the model mycobacterium <i>Mycobacterium smegmatis</i> to study the requirement for individual Mce, Mam, and Omam proteins in Mce4 transport of cholesterol. All of the Mce4 and Mam4 proteins we investigated were required for 2021-01-01T00:00:00Z2021 Apr2026-05-08T15:54:00.203Z2024-11-21T07:36:14.104ZS-EPMC80886073364915010.1128/jb.00685-2010.1128/JB.00685-20