{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang X"],"funding":["National Natural Science Foundation of China"],"pagination":["151"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8088648"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(1)"],"pubmed_abstract":["Background
Ovarian cancer is highly lethal and has a poor prognosis due to metastasis. Long non-coding RNAs (lncRNAs) are key regulators of tumor development, but their role in ovarian cancer metastasis remains unclear.Methods
The expression of lnc-CTSLP8 in ovarian cancer was analyzed in public databases (TCGA and GEO) and validated via qRT-PCR. Lnc-CTSLP8 overexpression and knockout cell lines were constructed using a lentiviral vector and the CRISP/Cas9 system. Cell proliferation, colony formation, migration, and invasion were analyzed. An ovarian orthotopic tumor mouse model was used for the in vivo study. Changes in autophagosomes, autolysosomes, and mitochondria in ovarian cancer cells were observed via transmission electron microscopy. EMT markers were detected by im"],"journal":["Journal of experimental & clinical cancer research : CR"],"pubmed_title":["The lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA and promotes ovarian cancer metastasis."],"pmcid":["PMC8088648"],"funding_grant_id":["81874103","81872117","81930064","81602280"],"pubmed_authors":["Li X","Lin F","Lin Y","Wang X","Sun H","Wang Z"],"additional_accession":[]},"is_claimable":false,"name":"The lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA and promotes ovarian cancer metastasis.","description":"Background
Ovarian cancer is highly lethal and has a poor prognosis due to metastasis. Long non-coding RNAs (lncRNAs) are key regulators of tumor development, but their role in ovarian cancer metastasis remains unclear.Methods
The expression of lnc-CTSLP8 in ovarian cancer was analyzed in public databases (TCGA and GEO) and validated via qRT-PCR. Lnc-CTSLP8 overexpression and knockout cell lines were constructed using a lentiviral vector and the CRISP/Cas9 system. Cell proliferation, colony formation, migration, and invasion were analyzed. An ovarian orthotopic tumor mouse model was used for the in vivo study. Changes in autophagosomes, autolysosomes, and mitochondria in ovarian cancer cells were observed via transmission electron microscopy. EMT markers were detected by im","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-05-09T00:21:50.418Z","creation":"2024-11-19T17:48:42.985Z"},"accession":"S-EPMC8088648","cross_references":{"pubmed":["33933142"],"doi":["10.1186/s13046-021-01957-z"]}}