biostudies-literatureLong SProgram for Top Scientific and Technological Talents in Guizhou ProvinceNatural Science Foundation of Guizhou ProvinceKey Program for Science and Technology of Guizhou ProvinceNational Natural Science Foundation of China185https://www.ebi.ac.uk/biostudies/studies/S-EPMC8088708biostudies-literatureUnknown19(1)<h4>Background</h4>Cetuximab has been approved for use for first-line treatment of patients with wild-type KRAS metastatic colorectal cancer (CRC). However, treatment with cetuximab has shown limited efficacy as a CRC monotherapy. In addition, natural killer (NK) cell function is known to be severely attenuated in cancer patients. The goal of this study was to develop a new strategy to enhance antibody-dependent cell-mediated cytotoxicity (ADCC) mediated by NK cells, in combination with cetuximab against CRC cells.<h4>Methods</h4>Ex vivo expanded NK cells were stimulated with reovirus, and reovirus-activated NK cells mediated ADCC assay were performed on CRC cells in combination with cetuximab. The synergistic antitumor effects of reovirus-activated NK cells and cetuximab were tested on DLJournal of translational medicineReovirus enhances cytotoxicity of natural killer cells against colorectal cancer via TLR3 pathway.PMC8088708ZK(2021)012]81860542(2019)5663KY (2018)049Wang NOuyang WGu YHe ZAn YLin XChen XWang XLiao CLong SZhao XfalseReovirus enhances cytotoxicity of natural killer cells against colorectal cancer via TLR3 pathway.<h4>Background</h4>Cetuximab has been approved for use for first-line treatment of patients with wild-type KRAS metastatic colorectal cancer (CRC). However, treatment with cetuximab has shown limited efficacy as a CRC monotherapy. In addition, natural killer (NK) cell function is known to be severely attenuated in cancer patients. The goal of this study was to develop a new strategy to enhance antibody-dependent cell-mediated cytotoxicity (ADCC) mediated by NK cells, in combination with cetuximab against CRC cells.<h4>Methods</h4>Ex vivo expanded NK cells were stimulated with reovirus, and reovirus-activated NK cells mediated ADCC assay were performed on CRC cells in combination with cetuximab. The synergistic antitumor effects of reovirus-activated NK cells and cetuximab were tested on DL2021-01-01T00:00:00Z2021 May2026-05-08T15:56:50.685Z2025-02-19T03:43:36.819ZS-EPMC80887083393313210.1186/s12967-021-02853-y