{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kaneko Y"],"funding":["Ministry of Education, Culture, Sports, Science and Technology"],"pagination":["e105853"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8090856"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(9)"],"pubmed_abstract":["p97ATPase-mediated membrane fusion is required for the biogenesis of the Golgi complex. p97 and its cofactor p47 function in soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) priming, but the tethering complex for p97/p47-mediated membrane fusion remains unknown. In this study, we identified formiminotransferase cyclodeaminase (FTCD) as a novel p47-binding protein. FTCD mainly localizes to the Golgi complex and binds to either p47 or p97 via its association with their polyglutamate motifs. FTCD functions in p97/p47-mediated Golgi reassembly at mitosis in vivo and in vitro via its binding to p47 and to p97. We also showed that FTCD, p47, and p97 form a big FTCD-p97/p47-FTCD tethering complex. In vivo tethering assay revealed that FTCD that was designed to l"],"journal":["The EMBO journal"],"pubmed_title":["p97 and p47 function in membrane tethering in cooperation with FTCD during mitotic Golgi reassembly."],"pmcid":["PMC8090856"],"funding_grant_id":["16K07353"],"pubmed_authors":["Kaneko Y","Goto Y","Zhang X","Kondo H","Ayala R","Shimoda K","Panico S"],"additional_accession":[]},"is_claimable":false,"name":"p97 and p47 function in membrane tethering in cooperation with FTCD during mitotic Golgi reassembly.","description":"p97ATPase-mediated membrane fusion is required for the biogenesis of the Golgi complex. p97 and its cofactor p47 function in soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) priming, but the tethering complex for p97/p47-mediated membrane fusion remains unknown. In this study, we identified formiminotransferase cyclodeaminase (FTCD) as a novel p47-binding protein. FTCD mainly localizes to the Golgi complex and binds to either p47 or p97 via its association with their polyglutamate motifs. FTCD functions in p97/p47-mediated Golgi reassembly at mitosis in vivo and in vitro via its binding to p47 and to p97. We also showed that FTCD, p47, and p97 form a big FTCD-p97/p47-FTCD tethering complex. In vivo tethering assay revealed that FTCD that was designed to l","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2026-06-01T03:08:40.508Z","creation":"2025-04-05T19:57:24.673Z"},"accession":"S-EPMC8090856","cross_references":{"pubmed":["33555040"],"doi":["10.15252/embj.2020105853"]}}