biostudies-literatureKaneko YMinistry of Education, Culture, Sports, Science and Technologye105853https://www.ebi.ac.uk/biostudies/studies/S-EPMC8090856biostudies-literatureUnknown40(9)p97ATPase-mediated membrane fusion is required for the biogenesis of the Golgi complex. p97 and its cofactor p47 function in soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) priming, but the tethering complex for p97/p47-mediated membrane fusion remains unknown. In this study, we identified formiminotransferase cyclodeaminase (FTCD) as a novel p47-binding protein. FTCD mainly localizes to the Golgi complex and binds to either p47 or p97 via its association with their polyglutamate motifs. FTCD functions in p97/p47-mediated Golgi reassembly at mitosis in vivo and in vitro via its binding to p47 and to p97. We also showed that FTCD, p47, and p97 form a big FTCD-p97/p47-FTCD tethering complex. In vivo tethering assay revealed that FTCD that was designed to lThe EMBO journalp97 and p47 function in membrane tethering in cooperation with FTCD during mitotic Golgi reassembly.PMC809085616K07353Kaneko YGoto YZhang XKondo HAyala RShimoda KPanico Sfalsep97 and p47 function in membrane tethering in cooperation with FTCD during mitotic Golgi reassembly.p97ATPase-mediated membrane fusion is required for the biogenesis of the Golgi complex. p97 and its cofactor p47 function in soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) priming, but the tethering complex for p97/p47-mediated membrane fusion remains unknown. In this study, we identified formiminotransferase cyclodeaminase (FTCD) as a novel p47-binding protein. FTCD mainly localizes to the Golgi complex and binds to either p47 or p97 via its association with their polyglutamate motifs. FTCD functions in p97/p47-mediated Golgi reassembly at mitosis in vivo and in vitro via its binding to p47 and to p97. We also showed that FTCD, p47, and p97 form a big FTCD-p97/p47-FTCD tethering complex. In vivo tethering assay revealed that FTCD that was designed to l2021-01-01T00:00:00Z2021 May2026-06-01T03:08:40.508Z2025-04-05T19:57:24.673ZS-EPMC80908563355504010.15252/embj.2020105853