{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Broto L"],"funding":["Ministerio de Ciencia, Innovación y Universidades","MEC | Agencia Estatal de Investigación","Comunidad Autónoma de Madrid","Ministerio de Economía y Competitividad","Caixa Foundation"],"pagination":["JVI.02017-20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8092823"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["95(5)"],"pubmed_abstract":["Infectious bursal disease virus (IBDV), the best characterized member of the <i>Birnaviridae</i> family, is a highly relevant avian pathogen causing both acute and persistent infections in different avian hosts. Here, we describe the establishment of clonal, long-term, productive persistent IBDV infections in DF-1 chicken embryonic fibroblasts. Although virus yields in persistently-infected cells are exceedingly lower than those detected in acutely infected cells, the replication fitness of viruses isolated from persistently-infected cells is higher than that of the parental virus. Persistently-infected DF-1 and IBDV-cured cell lines derived from them do not respond to type I interferon (IFN). High-throughput genome sequencing revealed that this defect is due to mutations affecting the IFN"],"journal":["Journal of virology"],"pubmed_title":["Type I Interferon acts as a major barrier to the establishment of infectious bursal disease virus (IBDV) persistent infections."],"pmcid":["PMC8092823"],"funding_grant_id":["FPU 18/01873","PEJD-2017-PRE/BIO-5006","AGL2017-87464-C2-1-P","AGL2014-60095-P","BES-2015-073589"],"pubmed_authors":["Candelas-Rivera O","Rodriguez JR","Diaz-Beneitez E","Rodriguez D","Eterradossi N","Broto L","Fuentes D","Cubas-Gaona LL","Soubies SM","Romero N","Courtillon C","Mendez F","Rodriguez JF"],"additional_accession":[]},"is_claimable":false,"name":"Type I Interferon acts as a major barrier to the establishment of infectious bursal disease virus (IBDV) persistent infections.","description":"Infectious bursal disease virus (IBDV), the best characterized member of the <i>Birnaviridae</i> family, is a highly relevant avian pathogen causing both acute and persistent infections in different avian hosts. Here, we describe the establishment of clonal, long-term, productive persistent IBDV infections in DF-1 chicken embryonic fibroblasts. Although virus yields in persistently-infected cells are exceedingly lower than those detected in acutely infected cells, the replication fitness of viruses isolated from persistently-infected cells is higher than that of the parental virus. Persistently-infected DF-1 and IBDV-cured cell lines derived from them do not respond to type I interferon (IFN). High-throughput genome sequencing revealed that this defect is due to mutations affecting the IFN","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-26T00:50:47.276Z","creation":"2022-02-11T05:51:44.354Z"},"accession":"S-EPMC8092823","cross_references":{"pubmed":["33328313"],"doi":["10.1128/jvi.02017-20","10.1128/JVI.02017-20"]}}