biostudies-literatureBroto LMinisterio de Ciencia, Innovación y UniversidadesMEC | Agencia Estatal de InvestigaciónComunidad Autónoma de MadridMinisterio de Economía y CompetitividadCaixa FoundationJVI.02017-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC8092823biostudies-literatureUnknown95(5)Infectious bursal disease virus (IBDV), the best characterized member of the <i>Birnaviridae</i> family, is a highly relevant avian pathogen causing both acute and persistent infections in different avian hosts. Here, we describe the establishment of clonal, long-term, productive persistent IBDV infections in DF-1 chicken embryonic fibroblasts. Although virus yields in persistently-infected cells are exceedingly lower than those detected in acutely infected cells, the replication fitness of viruses isolated from persistently-infected cells is higher than that of the parental virus. Persistently-infected DF-1 and IBDV-cured cell lines derived from them do not respond to type I interferon (IFN). High-throughput genome sequencing revealed that this defect is due to mutations affecting the IFNJournal of virologyType I Interferon acts as a major barrier to the establishment of infectious bursal disease virus (IBDV) persistent infections.PMC8092823FPU 18/01873PEJD-2017-PRE/BIO-5006AGL2017-87464-C2-1-PAGL2014-60095-PBES-2015-073589Candelas-Rivera ORodriguez JRDiaz-Beneitez ERodriguez DEterradossi NBroto LFuentes DCubas-Gaona LLSoubies SMRomero NCourtillon CMendez FRodriguez JFfalseType I Interferon acts as a major barrier to the establishment of infectious bursal disease virus (IBDV) persistent infections.Infectious bursal disease virus (IBDV), the best characterized member of the <i>Birnaviridae</i> family, is a highly relevant avian pathogen causing both acute and persistent infections in different avian hosts. Here, we describe the establishment of clonal, long-term, productive persistent IBDV infections in DF-1 chicken embryonic fibroblasts. Although virus yields in persistently-infected cells are exceedingly lower than those detected in acutely infected cells, the replication fitness of viruses isolated from persistently-infected cells is higher than that of the parental virus. Persistently-infected DF-1 and IBDV-cured cell lines derived from them do not respond to type I interferon (IFN). High-throughput genome sequencing revealed that this defect is due to mutations affecting the IFN2021-01-01T00:00:00Z2021 Mar2025-04-26T00:50:47.276Z2022-02-11T05:51:44.354ZS-EPMC80928233332831310.1128/jvi.02017-2010.1128/JVI.02017-20