biostudies-literatureValcek ACharles University Research Fund PROGRESMinistry of Education Youth and Sports of the Czech RepublicNational Sustainability Program IHorizon 2020OH-EJP-H2020-JRP-AMR-2-ARDIGInternal Grant Agency of University of Veterinary and Pharmaceutical Sciences BrnoCzech Science FoundationUtrecht UniversityAAC.02556-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC8092906biostudies-literatureUnknown65(5)The relatedness of the equine-associated <i>Escherichia coli</i> ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of <i>E. coli</i> ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe [Czech Republic (n=23), the Netherlands (n=18), Germany (n=9), Denmark (n=3) and France (n=1)] from 2008-2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of <i>E. coli</i> ST1250 and ST1250-SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 and one IncHI1/ST2 plasmids were obtaiAntimicrobial agents and chemotherapyHorsing around: <i>Escherichia coli</i> ST1250 of equine origin harbouring epidemic IncHI1/ST9 plasmid with <i>bla</i> _CTX-M-1 and an operon for short-chain fructooligosaccharides metabolism.PMC8092906116/2015/FVLCZ.02.1.01/0.0/0.0/16_019/0000787204/2020/FVHEQ39LO150377383018-23532SBrouwer MSMJamborova IWagenaar JAvan Duijkeren EValcek AHrabak JSismova PDamborg PEwers CHasman HNesporova KOverballe-Petersen SMadec JYDolejska MKant AHordijk JBitar IfalseHorsing around: <i>Escherichia coli</i> ST1250 of equine origin harbouring epidemic IncHI1/ST9 plasmid with <i>bla</i> _CTX-M-1 and an operon for short-chain fructooligosaccharides metabolism.The relatedness of the equine-associated <i>Escherichia coli</i> ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of <i>E. coli</i> ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe [Czech Republic (n=23), the Netherlands (n=18), Germany (n=9), Denmark (n=3) and France (n=1)] from 2008-2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of <i>E. coli</i> ST1250 and ST1250-SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 and one IncHI1/ST2 plasmids were obtai2023-01-01T00:00:00Z2023 May2026-05-07T22:14:55.266Z2024-11-08T20:59:46.721ZS-EPMC80929063361906310.1128/aac.02556-2010.1128/AAC.02556-20