{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Yang HQ"],"pubmed_abstract":["T cell responses play critical roles in host adaptive immunity against <i>Pneumocystis</i>. However, the dynamics and diversity of the T cell immune repertoire in human immunodeficiency virus (HIV)-negative <i>Pneumocystis</i> pneumonia (PCP) remains unclear. In this study, single-cell RNA and single-cell T cell receptor (TCR) sequencing were applied to cells sorted from lung tissues of mice infected with <i>Pneumocystis</i>. Our findings demonstrated the clonal cells were mainly composed of CD4<sup>+</sup> T cells in response to <i>Pneumocystis</i>, which were marked by highly expressed genes associated with T cell activation. Mice infected with <i>Pneumocystis</i> showed reduced TCR diversity in CD4<sup>+</sup> T cells and increased diversity in CD8<sup>+</sup> T cells compared with uninfected controls. Furthermore, Th17 cells were mostly clonal CD4<sup>+</sup> T cells, which exhibited the phenotype of tissue-resident memory-like Th17 cells. In addition, <i>Pneumocystis</i>-infected mice showed biased usage of TCRβ VDJ genes. Taken together, we characterized the transcriptome and TCR immune repertoires profiles of expanded T cell clones, which demonstrate a skewed TCR repertoire after <i>Pneumocystis</i> infection."],"journal":["Frontiers in microbiology"],"pagination":["637500"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8093776"],"repository":["biostudies-literature"],"pubmed_title":["Single-Cell TCR Sequencing Reveals the Dynamics of T Cell Repertoire Profiling During <i>Pneumocystis</i> Infection."],"pmcid":["PMC8093776"],"pubmed_authors":["Yang HQ","Wang YS","Tong ZH","Zhai K"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell TCR Sequencing Reveals the Dynamics of T Cell Repertoire Profiling During <i>Pneumocystis</i> Infection.","description":"T cell responses play critical roles in host adaptive immunity against <i>Pneumocystis</i>. However, the dynamics and diversity of the T cell immune repertoire in human immunodeficiency virus (HIV)-negative <i>Pneumocystis</i> pneumonia (PCP) remains unclear. In this study, single-cell RNA and single-cell T cell receptor (TCR) sequencing were applied to cells sorted from lung tissues of mice infected with <i>Pneumocystis</i>. Our findings demonstrated the clonal cells were mainly composed of CD4<sup>+</sup> T cells in response to <i>Pneumocystis</i>, which were marked by highly expressed genes associated with T cell activation. Mice infected with <i>Pneumocystis</i> showed reduced TCR diversity in CD4<sup>+</sup> T cells and increased diversity in CD8<sup>+</sup> T cells compared with uninfected controls. Furthermore, Th17 cells were mostly clonal CD4<sup>+</sup> T cells, which exhibited the phenotype of tissue-resident memory-like Th17 cells. In addition, <i>Pneumocystis</i>-infected mice showed biased usage of TCRβ VDJ genes. Taken together, we characterized the transcriptome and TCR immune repertoires profiles of expanded T cell clones, which demonstrate a skewed TCR repertoire after <i>Pneumocystis</i> infection.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-04-07T17:01:26.394Z","creation":"2025-02-19T03:43:13.199Z"},"accession":"S-EPMC8093776","cross_references":{"pubmed":["33959105"],"doi":["10.3389/fmicb.2021.637500"]}}